CTK1/YKL139W Protein Information

Standard Name	Ctk1p 1
Systematic Name	Ykl139wp
ORF Classification	Verified
Description	Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 (1, 2, 3, 4)
Name Description	Carboxy-Terminal domain Kinase 1

Experimental Data
Molecules/cell	125 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	528
Molecular Weight (Da)	60,501
Isoelectric Point (pI)	10.3

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ctk1p (InterPro)
Physical Interactions	There are 100 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005323B \| Q03957 MIPS: YKL139W NCBI: 171328 \| 416848 \| 486235 \| 6322710 \| NP_012783.1 \| NM_001179705.1 GenBank/EMBL/DDBJ: DAA09023.1 \| M69024 \| Z28139
External Classifications	EC: 2.7.11.23 [[RNA-polymerase]-subunit kinase]

Amino Acid Sequence (or in FASTA format)
1 MSYNNGNTYS KSYSRNNKRP LFGKRSPNPQ SLARPPPPKR IRTDSGYQSN 51 MDNISSHRVN SNDQPGHTKS RGNNNLSRYN DTSFQTSSRY QGSRYNNNNT 101 SYENRPKSIK RDETKAEFLS HLPKGPKSVE KSRYNNSSNT SNDIKNGYHA 151 SKYYNHKGQE GRSVIAKKVP VSVLTQQRST SVYLRIMQVG EGTYGKVYKA 201 KNTNTEKLVA LKKLRLQGER EGFPITSIRE IKLLQSFDHP NVSTIKEIMV 251 ESQKTVYMIF EYADNDLSGL LLNKEVQISH SQCKHLFKQL LLGMEYLHDN 301 KILHRDVKGS NILIDNQGNL KITDFGLARK MNSRADYTNR VITLWYRPPE 351 LLLGTTNYGT EVDMWGCGCL LVELFNKTAI FQGSNELEQI ESIFKIMGTP 401 TINSWPTLYD MPWFFMIMPQ QTTKYVNNFS EKFKSVLPSS KCLQLAINLL 451 CYDQTKRFSA TEALQSDYFK EEPKPEPLVL DGLVSCHEYE VKLARKQKRP 501 NILSTNTNNK GNGNSNNNNN NNNDDDDK*

Amino Acid Sequence (or in FASTA format)

       1  MSYNNGNTYS KSYSRNNKRP LFGKRSPNPQ SLARPPPPKR IRTDSGYQSN
      51  MDNISSHRVN SNDQPGHTKS RGNNNLSRYN DTSFQTSSRY QGSRYNNNNT
     101  SYENRPKSIK RDETKAEFLS HLPKGPKSVE KSRYNNSSNT SNDIKNGYHA
     151  SKYYNHKGQE GRSVIAKKVP VSVLTQQRST SVYLRIMQVG EGTYGKVYKA
     201  KNTNTEKLVA LKKLRLQGER EGFPITSIRE IKLLQSFDHP NVSTIKEIMV
     251  ESQKTVYMIF EYADNDLSGL LLNKEVQISH SQCKHLFKQL LLGMEYLHDN
     301  KILHRDVKGS NILIDNQGNL KITDFGLARK MNSRADYTNR VITLWYRPPE
     351  LLLGTTNYGT EVDMWGCGCL LVELFNKTAI FQGSNELEQI ESIFKIMGTP
     401  TINSWPTLYD MPWFFMIMPQ QTTKYVNNFS EKFKSVLPSS KCLQLAINLL
     451  CYDQTKRFSA TEALQSDYFK EEPKPEPLVL DGLVSCHEYE VKLARKQKRP
     501  NILSTNTNNK GNGNSNNNNN NNNDDDDK*

external links for Ctk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ctk1p

1)	Lee JM and Greenleaf AL (1991) CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae. Gene Expr 1(2):149-67
2)	Skaar DA and Greenleaf AL (2002) The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. Mol Cell 10(6):1429-39
3)	Rother S and Strasser K (2007) The RNA polymerase II CTD kinase Ctk1 functions in translation elongation. Genes Dev 21(11):1409-21
4)	Bartkowiak B, et al. (2010) CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. Genes Dev 24(20):2303-16
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41