SHE2/YKL130C Protein Information

Standard Name	She2p 1
Systematic Name	Ykl130cp
ORF Classification	Verified
Description	RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud (2, 3, 4, 5, 6)
Name Description	Swi5p-dependent HO Expression 1

Experimental Data
Molecules/cell	4070 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	246
Molecular Weight (Da)	28,251
Isoelectric Point (pI)	4.7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with She2p (InterPro)
Physical Interactions	There are 128 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FED \| P36068 MIPS: YKL130C NCBI: 45269695 \| 486221 \| 549715 \| 56967134 \| 56967135 \| 6322719 \| NP_012792.1 GenBank/EMBL/DDBJ: DAA09031.1 \| AY557902 \| Z28130

Amino Acid Sequence (or in FASTA format)
1 MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF 51 ERTTLIKFVK KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA 101 SMFVKCVETF DLLNYYLTQS LQKEILSKTL NEDLTLTAES ILAIDDTYNH 151 FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC ADEDGTNIGE TDNIFLQEIL 201 PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK FGKLKN*

Amino Acid Sequence (or in FASTA format)

       1  MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF
      51  ERTTLIKFVK KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA
     101  SMFVKCVETF DLLNYYLTQS LQKEILSKTL NEDLTLTAES ILAIDDTYNH
     151  FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC ADEDGTNIGE TDNIFLQEIL
     201  PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK FGKLKN*

external links for She2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for She2p

1)	Jansen RP, et al. (1996) Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins. Cell 84(5):687-97
2)	Takizawa PA and Vale RD (2000) The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Proc Natl Acad Sci U S A 97(10):5273-8
3)	Long RM, et al. (2000) She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA. EMBO J 19(23):6592-601
4)	Bohl F, et al. (2000) She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p. EMBO J 19(20):5514-24
5)	Shepard KA, et al. (2003) Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A 100(20):11429-34
6)	Estrada P, et al. (2003) Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae. J Cell Biol 163(6):1255-66
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41