YPK1/YKL126W Protein Information

Standard Name	Ypk1p
Systematic Name	Ykl126wp
Alias	Sli2p
ORF Classification	Verified
Description	Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description	Yeast Protein Kinase

Experimental Data
Molecules/cell	2950 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	680
Molecular Weight (Da)	76,479
Isoelectric Point (pI)	6.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ypk1p (InterPro)
Physical Interactions	There are 40 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BDFE \| P12688 MIPS: YKL126W NCBI: 172181 \| 486213 \| 549804 \| 6322723 \| NP_012796.1 \| NM_001179692.1 GenBank/EMBL/DDBJ: DAA09035.1 \| M21307 \| Z28126
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MYSWKSKFKF GKSKEEKEAK HSGFFHSSKK EEQQNNQATA GEHDASITRS 51 SLDRKGTINP SNSSVVPVRV SYDASSSTST VRDSNGGNSE NTNSSQNLDE 101 TANIGSTGTP NDATSSSGMM TIKVYNGDDF ILPFPITSSE QILNKLLASG 151 VPPPHKEISK EVDALIAQLS RVQIKNQGPA DEDLISSESA AKFIPSTIML 201 PGSSTLNPLL YFTIEFDNTV ATIEAEYGTI AKPGFNKIST FDVTRKLPYL 251 KIDVFARIPS ILLPSKTWQQ EMGLQDEKLQ TIFDKINSNQ DIHLDSFHLP 301 INLSFDSAAS IRLYNHHWIT LDNGLGKINI SIDYKPSRNK PLSIDDFDLL 351 KVIGKGSFGK VMQVRKKDTQ KVYALKAIRK SYIVSKSEVT HTLAERTVLA 401 RVDCPFIVPL KFSFQSPEKL YFVLAFINGG ELFYHLQKEG RFDLSRARFY 451 TAELLCALDN LHKLDVVYRD LKPENILLDY QGHIALCDFG LCKLNMKDDD 501 KTDTFCGTPE YLAPELLLGL GYTKAVDWWT LGVLLYEMLT GLPPYYDEDV 551 PKMYKKILQE PLVFPDGFDR DAKDLLIGLL SRDPTRRLGY NGADEIRNHP 601 FFSQLSWKRL LMKGYIPPYK PAVSNSMDTS NFDEEFTREK PIDSVVDEYL 651 SESVQKQFGG WTYVGNEQLG SSMVQGRSIR *

Amino Acid Sequence (or in FASTA format)

       1  MYSWKSKFKF GKSKEEKEAK HSGFFHSSKK EEQQNNQATA GEHDASITRS
      51  SLDRKGTINP SNSSVVPVRV SYDASSSTST VRDSNGGNSE NTNSSQNLDE
     101  TANIGSTGTP NDATSSSGMM TIKVYNGDDF ILPFPITSSE QILNKLLASG
     151  VPPPHKEISK EVDALIAQLS RVQIKNQGPA DEDLISSESA AKFIPSTIML
     201  PGSSTLNPLL YFTIEFDNTV ATIEAEYGTI AKPGFNKIST FDVTRKLPYL
     251  KIDVFARIPS ILLPSKTWQQ EMGLQDEKLQ TIFDKINSNQ DIHLDSFHLP
     301  INLSFDSAAS IRLYNHHWIT LDNGLGKINI SIDYKPSRNK PLSIDDFDLL
     351  KVIGKGSFGK VMQVRKKDTQ KVYALKAIRK SYIVSKSEVT HTLAERTVLA
     401  RVDCPFIVPL KFSFQSPEKL YFVLAFINGG ELFYHLQKEG RFDLSRARFY
     451  TAELLCALDN LHKLDVVYRD LKPENILLDY QGHIALCDFG LCKLNMKDDD
     501  KTDTFCGTPE YLAPELLLGL GYTKAVDWWT LGVLLYEMLT GLPPYYDEDV
     551  PKMYKKILQE PLVFPDGFDR DAKDLLIGLL SRDPTRRLGY NGADEIRNHP
     601  FFSQLSWKRL LMKGYIPPYK PAVSNSMDTS NFDEEFTREK PIDSVVDEYL
     651  SESVQKQFGG WTYVGNEQLG SSMVQGRSIR *

external links for Ypk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ypk1p

1)	Sun Y, et al. (2000) Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Mol Cell Biol 20(12):4411-9
2)	Casamayor A, et al. (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
3)	deHart AK, et al. (2002) The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast. J Cell Biol 156(2):241-8
4)	Schmelzle T, et al. (2002) Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol 22(5):1329-39
5)	Roelants FM, et al. (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Roelants FM, et al. (2010) A protein kinase network regulates the function of aminophospholipid flippases. Proc Natl Acad Sci U S A 107(1):34-9
8)	Roelants FM, et al. (2011) Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 108(48):19222-7
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41