RRN3/YKL125W Protein Information

Standard Name	Rrn3p 1
Systematic Name	Ykl125wp
ORF Classification	Verified
Description	Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo (2, 3, 4)
Name Description	Regulation of RNA polymerase I 1

Experimental Data
Molecules/cell	138 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	627
Molecular Weight (Da)	72,387
Isoelectric Point (pI)	4.42

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rrn3p (InterPro)
Physical Interactions	There are 21 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005324F \| P36070 MIPS: YKL125W NCBI: 1403568 \| 347948697 \| 347948698 \| 486211 \| 549712 \| 6322724 \| NP_012797.1 \| NM_001179691.1 GenBank/EMBL/DDBJ: DAA09036.1 \| Z28125 \| Z71927

Amino Acid Sequence (or in FASTA format)
1 MMAFENTSKR PPQDFVAPID QKKRKVQFSD STGLVTLQPE EIKDEVFSAA 51 MYSRFVKSAL DDLDKNDSTQ IGIIANQVAL PSKNPERIND KNLNILLDIL 101 SSNINRIESS RGTFLIQSII NFEKWWELPP HTLSKYIYFI KILCSSIPKW 151 WQDVSMILVS CFILPIKQTV CHHDMLKYFL RMIPSSMGFI DTYLAKFFPN 201 KNDTRRKLVN YTSNLLKLRG YCSELGFQIW SLLIEKIISI DVELQNELDE 251 LDDDVDDDDL EEVDLEDDDD LDDDSGDDDD ENCGNSNEEL RSGAADGSQS 301 DSEDMDIIEG MDGTEEYNVE LTQGIKELST KLDSILTLVS THVEEQVTPE 351 SLESGEGVGV FNTLTTLFKT HVLPTYYTRS IQYIMFHVSQ QQLELMDSFL 401 VTLIDISFAV NEAAEKKIKS LQYLGSYIAR AKKLSRTQII FVASYLTSWL 451 NRYVIEREEE VDQRGGMERF KHFYAAFQAL CYIFCFRHNI FRDTDGNWEC 501 ELDKFFQRMV ISKFNPLKFC NENVMLMFAR IAQQESVAYC FSIIENNNNE 551 RLRGIIGKAD SDKKENSAQA NTTSSSWSLA TRQQFIDLQS YFPYDPLFLK 601 NYKILMKEYY IEWSEASGEY ESDGSDD*

Amino Acid Sequence (or in FASTA format)

       1  MMAFENTSKR PPQDFVAPID QKKRKVQFSD STGLVTLQPE EIKDEVFSAA
      51  MYSRFVKSAL DDLDKNDSTQ IGIIANQVAL PSKNPERIND KNLNILLDIL
     101  SSNINRIESS RGTFLIQSII NFEKWWELPP HTLSKYIYFI KILCSSIPKW
     151  WQDVSMILVS CFILPIKQTV CHHDMLKYFL RMIPSSMGFI DTYLAKFFPN
     201  KNDTRRKLVN YTSNLLKLRG YCSELGFQIW SLLIEKIISI DVELQNELDE
     251  LDDDVDDDDL EEVDLEDDDD LDDDSGDDDD ENCGNSNEEL RSGAADGSQS
     301  DSEDMDIIEG MDGTEEYNVE LTQGIKELST KLDSILTLVS THVEEQVTPE
     351  SLESGEGVGV FNTLTTLFKT HVLPTYYTRS IQYIMFHVSQ QQLELMDSFL
     401  VTLIDISFAV NEAAEKKIKS LQYLGSYIAR AKKLSRTQII FVASYLTSWL
     451  NRYVIEREEE VDQRGGMERF KHFYAAFQAL CYIFCFRHNI FRDTDGNWEC
     501  ELDKFFQRMV ISKFNPLKFC NENVMLMFAR IAQQESVAYC FSIIENNNNE
     551  RLRGIIGKAD SDKKENSAQA NTTSSSWSLA TRQQFIDLQS YFPYDPLFLK
     601  NYKILMKEYY IEWSEASGEY ESDGSDD*

external links for Rrn3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rrn3p

1)	Nogi Y, et al. (1991) An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(16):7026-30
2)	Yamamoto RT, et al. (1996) RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template. EMBO J 15(15):3964-73
3)	Peyroche G, et al. (2000) The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J 19(20):5473-82
4)	Blattner C, et al. (2011) Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth. Genes Dev 25(19):2093-105
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41