RAD27/YKL113C Protein Information

Standard Name	Rad27p 1
Systematic Name	Ykl113cp
Alias	Erc11p , Rth1p 2 , Fen1p 3
ORF Classification	Verified
Description	5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family (4, 5)
Name Description	RADiation sensitive 1

Experimental Data
Molecules/cell	6120 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	382
Molecular Weight (Da)	43,279
Isoelectric Point (pI)	9.51

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rad27p (InterPro)
Physical Interactions	There are 22 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000050E10 \| P26793 MIPS: YKL113C NCBI: 140964 \| 248392 \| 486190 \| 6322736 \| NP_012809.1 GenBank/EMBL/DDBJ: DAA09045.1 \| S93804 \| Z28113

Amino Acid Sequence (or in FASTA format)
1 MGIKGLNAII SEHVPSAIRK SDIKSFFGRK VAIDASMSLY QFLIAVRQQD 51 GGQLTNEAGE TTSHLMGMFY RTLRMIDNGI KPCYVFDGKP PDLKSHELTK 101 RSSRRVETEK KLAEATTELE KMKQERRLVK VSKEHNEEAQ KLLGLMGIPY 151 IIAPTEAEAQ CAELAKKGKV YAAASEDMDT LCYRTPFLLR HLTFSEAKKE 201 PIHEIDTELV LRGLDLTIEQ FVDLCIMLGC DYCESIRGVG PVTALKLIKT 251 HGSIEKIVEF IESGESNNTK WKIPEDWPYK QARMLFLDPE VIDGNEINLK 301 WSPPKEKELI EYLCDDKKFS EERVKSGISR LKKGLKSGIQ GRLDGFFQVV 351 PKTKEQLAAA AKRAQENKKL NKNKNKVTKG RR*

Amino Acid Sequence (or in FASTA format)

       1  MGIKGLNAII SEHVPSAIRK SDIKSFFGRK VAIDASMSLY QFLIAVRQQD
      51  GGQLTNEAGE TTSHLMGMFY RTLRMIDNGI KPCYVFDGKP PDLKSHELTK
     101  RSSRRVETEK KLAEATTELE KMKQERRLVK VSKEHNEEAQ KLLGLMGIPY
     151  IIAPTEAEAQ CAELAKKGKV YAAASEDMDT LCYRTPFLLR HLTFSEAKKE
     201  PIHEIDTELV LRGLDLTIEQ FVDLCIMLGC DYCESIRGVG PVTALKLIKT
     251  HGSIEKIVEF IESGESNNTK WKIPEDWPYK QARMLFLDPE VIDGNEINLK
     301  WSPPKEKELI EYLCDDKKFS EERVKSGISR LKKGLKSGIQ GRLDGFFQVV
     351  PKTKEQLAAA AKRAQENKKL NKNKNKVTKG RR*

external links for Rad27p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rad27p

1)	Reagan MS, et al. (1995) Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene. J Bacteriol 177(2):364-71
2)	Sommers CH, et al. (1995) Conditional lethality of null mutations in RTH1 that encodes the yeast counterpart of a mammalian 5'- to 3'-exonuclease required for lagging strand DNA synthesis in reconstituted systems. J Biol Chem 270(9):4193-6
3)	Gary R, et al. (1999) A novel role in DNA metabolism for the binding of Fen1/Rad27 to PCNA and implications for genetic risk. Mol Cell Biol 19(8):5373-82
4)	Kao HI, et al. (2002) Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate. J Biol Chem 277(17):14379-89
5)	Ayyagari R, et al. (2003) Okazaki fragment maturation in yeast. I. Distribution of functions between FEN1 AND DNA2. J Biol Chem 278(3):1618-25
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41