KTI12/YKL110C Protein Information Help

Standard Name Kti12p 1
Systematic Name Ykl110cp
Alias Tot4p 2
ORF Classification Verified
Description Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p (1, 3, 4, 5)
Name Description Kluveromyces lactis Toxin Insensitive 6
Experimental Data
Molecules/cell 5260 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 313
Molecular Weight (Da) 35,322
Isoelectric Point (pI) 7.98

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Kti12p (InterPro)
Physical Interactions There are 34 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012E0E0 | P34253
MIPS: YKL110C
NCBI: 1680182 | 431206 | 486185 | 536816 | 549706 | 6322739 | 806317 | NP_012812.1 | NM_001179676.1
GenBank/EMBL/DDBJ: AAA66312.1 | AAB22001.1 | CAA50447.1 | CAA54646.1 | CAA81950.1 | DAA09047.1 | M29067 | S93804 | X71133 | X77511 | Z28110
Amino Acid Sequence (or in FASTA format)
       1  MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI
      51  KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE
     101  VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS
     151  NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK
     201  PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI
     251  VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI
     301  GPLFADYLNK NLN*                                       

external links for Kti12p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Kti12p
1) Butler AR, et al.  (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
2) Frohloff F, et al.  (2001) Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J 20(8):1993-2003
3) Fichtner L, et al.  (2002) Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity. Mol Microbiol 45(3):817-26
4) Huang B, et al.  (2005) An early step in wobble uridine tRNA modification requires the Elongator complex. RNA 11(4):424-36
5) Petrakis TG, et al.  (2005) Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein. J Biol Chem 280(20):19454-60
6) Butler AR, et al.  (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41