KTI12/YKL110C Protein Information

Standard Name	Kti12p 1
Systematic Name	Ykl110cp
Alias	Tot4p 2
ORF Classification	Verified
Description	Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p (1, 3, 4, 5)
Name Description	Kluveromyces lactis Toxin Insensitive 6

Experimental Data
Molecules/cell	5260 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	313
Molecular Weight (Da)	35,322
Isoelectric Point (pI)	7.98

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Kti12p (InterPro)
Physical Interactions	There are 34 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012E0E0 \| P34253 MIPS: YKL110C NCBI: 1680182 \| 431206 \| 486185 \| 536816 \| 549706 \| 6322739 \| 806317 \| NP_012812.1 GenBank/EMBL/DDBJ: DAA09047.1 \| M29067 \| S93804 \| X71133 \| X77511 \| Z28110

Amino Acid Sequence (or in FASTA format)
1 MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI 51 KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE 101 VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS 151 NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK 201 PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI 251 VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI 301 GPLFADYLNK NLN*

Amino Acid Sequence (or in FASTA format)

       1  MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI
      51  KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE
     101  VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS
     151  NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK
     201  PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI
     251  VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI
     301  GPLFADYLNK NLN*

external links for Kti12p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Kti12p

1)	Butler AR, et al. (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
2)	Frohloff F, et al. (2001) Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J 20(8):1993-2003
3)	Fichtner L, et al. (2002) Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity. Mol Microbiol 45(3):817-26
4)	Huang B, et al. (2005) An early step in wobble uridine tRNA modification requires the Elongator complex. RNA 11(4):424-36
5)	Petrakis TG, et al. (2005) Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein. J Biol Chem 280(20):19454-60
6)	Butler AR, et al. (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41