BUD2/YKL092C Protein Information Help

Standard Name Bud2p 1
Systematic Name Ykl092cp
Alias Cla2p , Erc25p
ORF Classification Verified
Description GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint (1, 2, 3)
Name Description BUD site selection
Experimental Data
Molecules/cell 1230 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,104
Molecular Weight (Da) 126,662
Isoelectric Point (pI) 9.33

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Bud2p (InterPro)
Physical Interactions There are 7 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000126B33 | P33314
MIPS: YKL092C
NCBI: 395289 | 402734 | 414689 | 486139 | 543907 | 6322758 | NP_012831.1
GenBank/EMBL/DDBJ: DAA09066.1 | L19162 | X74130 | X75561 | Z28092
Amino Acid Sequence (or in FASTA format)
 
       1  MSSNNEPAQS RTSYFKLNEF LSNVKHYKNT FKGEIQWCNN LSLNDWKTHY
      51  LQITSTGALT HSIDELTADS TNIQPIIKHL QQCRIEIIKD KHSSFKDINA
     101  NCNFIIQVNT SGKDNKVYLR VKSWSDFKKL LTCLIWWSSM KTNGIFNKFQ
     151  VSRPLEFKSK KMAKPESLLV YKLNVFGPIV KNIVLPPATN ILESPDIINN
     201  DDNSVGWFSA MGVLKSNGML DLLLQSDGSL IYSLNISQLL RSEIRILDSS
     251  VLQSENSLFL GELPLLRSQL GLEKFRIENI ASAATNSSDI SQEIIVEFPL
     301  RIDLEDCFIA LQSFARSEYL SITGSDKSND MKISNSFKIS ILEANFQSIN
     351  LNDKNNTPWS IFTDITAWGH TWARTSMVSN SSNPFWREEF QFNELLRLTN
     401  SYLEIKQLFH DLNNKKRLRL IGKIKITQEI INDTRYNKET RLPIMDVDNK
     451  NFQIGTICIK ISSNLNFILP STNFVKLEKL LMNANLSMVS NLIYKSSSSM
     501  ENDNKLTQTS IIFLDIFQSL SRIEEWFHVL IDKELAKIDG TVSRINQKNL
     551  DSKHVFNSLF RGNSILTKSI EQYFFRVGNE YLSKALSAIL KEIIESNKSC
     601  ELDPARVKEK DEVKKRKIIA DNYKRLYSWV TKIWKRLYAT SNDLPIEIRN
     651  VLKIFRQKLE IICIDDTLQI ILNGISGLLF LRFFCPVILN PKLFKYVSQN
     701  LNETARRNLT LISKVLLNLS TLTQFANKEP WLMKMNNFID KRHNDLLDYI
     751  DKMTQKKLDF NSKILNLSST ISRPKLAIEQ TMLDDLPQIP YLLDKNLRET
     801  EFVNLIVNFS QEDMTKMEKY NHMDNGGKGE LIEEEGLLSG SSLNLSVDKK
     851  DLDSPIEVKP EIGELEFEKI TENNTEIFGD DLMNLLKSDD VGSRSRDLDN
     901  GANSGIKFNS IIPKAEEEKH AMKELEQESC LLYNRINHIR KRLSGYECAS
     951  STLFEDKKYS ISLSHKIFYE EIKEGKEIVL KLLNKPTNEN SSARLQKFFT
    1001  KGVSSKSNNT VGDSYCKFLT IDVSDENPKS SNKTSVHGTS SENGAKDDYL
    1051  TLPNSQGKGN LGNRFSPTKL SRIMRKPPNA DVPKEQNSRK LTRWFKKKKE
    1101  TGGS*

external links for Bud2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Bud2p
1) Chant J and Herskowitz I  (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65(7):1203-12
2) Park HO, et al.  (1993) BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast. Nature 365(6443):269-74
3) Nelson SA, et al.  (2012) A Novel Role for the GTPase-Activating Protein Bud2 in the Spindle Position Checkpoint. PLoS One 7(4):e36127
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41