SMY1/YKL079W Protein Information

Standard Name	Smy1p 1
Systematic Name	Ykl079wp
ORF Classification	Verified
Description	Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins (2, 3)
Name Description	Suppressor of MYo2-66 1

Experimental Data
Molecules/cell	1920 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	656
Molecular Weight (Da)	73,799
Isoelectric Point (pI)	6.92

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Smy1p (InterPro)
Physical Interactions	There are 139 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135AF5 \| P32364 MIPS: YKL079W NCBI: 171100 \| 172626 \| 414715 \| 417786 \| 486114 \| 6322771 \| NP_012844.1 GenBank/EMBL/DDBJ: DAA09078.1 \| M69021 \| M77143 \| X75560 \| Z28079

Amino Acid Sequence (or in FASTA format)
1 MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID 51 PYENTVLFRT NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN 101 DVLNGYNGTV ITYGPSFSGK SYSLIGSKES EGILPNICKT LFDTLEKNEE 151 TKGDSFSVSV LAFEIYMEKT YDLLVPLPER KPLKLHRSSS KMDLEIKDIC 201 PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ LHVEQRNRKD 251 DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS 301 MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT 351 TLSTLEFGDS IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN 401 ILKAEIDSLK SLHNKSLPED DEKKMLENTK KENIKLKLQL DSITQLLSSS 451 TNEDPNNRID EEVSEILTKR CEQIAQLELS FDRQMNSNSK LQQELEYKKS 501 KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH TKLTETRSER 551 IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA 601 NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK 651 SPSSGS*

Amino Acid Sequence (or in FASTA format)

       1  MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID
      51  PYENTVLFRT NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN
     101  DVLNGYNGTV ITYGPSFSGK SYSLIGSKES EGILPNICKT LFDTLEKNEE
     151  TKGDSFSVSV LAFEIYMEKT YDLLVPLPER KPLKLHRSSS KMDLEIKDIC
     201  PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ LHVEQRNRKD
     251  DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS
     301  MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT
     351  TLSTLEFGDS IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN
     401  ILKAEIDSLK SLHNKSLPED DEKKMLENTK KENIKLKLQL DSITQLLSSS
     451  TNEDPNNRID EEVSEILTKR CEQIAQLELS FDRQMNSNSK LQQELEYKKS
     501  KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH TKLTETRSER
     551  IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA
     601  NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK
     651  SPSSGS*

external links for Smy1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Smy1p

1)	Lillie SH and Brown SS (1992) Suppression of a myosin defect by a kinesin-related gene. Nature 356(6367):358-61
2)	Beningo KA, et al. (2000) The yeast kinesin-related protein Smy1p exerts its effects on the class V myosin Myo2p via a physical interaction. Mol Biol Cell 11(2):691-702
3)	Lillie SH and Brown SS (1998) Smy1p, a kinesin-related protein that does not require microtubules. J Cell Biol 140(4):873-83
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41