NUP100/YKL068W Protein Information

Standard Name	Nup100p 1
Systematic Name	Ykl068wp
Alias	Nsp100p
ORF Classification	Verified
Description	FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication (10, 2, 3, 4, 5, 6, 7, 8, 9) Also known as: [NUP100+] 11
Name Description	NUclear Pore 12

Experimental Data
Molecules/cell	358 13

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	959
Molecular Weight (Da)	99,988
Isoelectric Point (pI)	10.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nup100p (InterPro)
Physical Interactions	There are 109 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012FC09 \| Q02629 MIPS: YKL068W NCBI: 400320 \| 4074 \| 433631 \| 486095 \| 6322782 \| NP_012855.1 GenBank/EMBL/DDBJ: DAA09089.1 \| X75780 \| Z15035 \| Z28068

Amino Acid Sequence (or in FASTA format)
1 MFGNNRPMFG GSNLSFGSNT SSFGGQQSQQ PNSLFGNSNN NNNSTSNNAQ 51 SGFGGFTSAA GSNSNSLFGN NNTQNNGAFG QSMGATQNSP FGSLNSSNAS 101 NGNTFGGSSS MGSFGGNTNN AFNNNSNSTN SPFGFNKPNT GGTLFGSQNN 151 NSAGTSSLFG GQSTSTTGTF GNTGSSFGTG LNGNGSNIFG AGNNSQSNTT 201 GSLFGNQQSS AFGTNNQQGS LFGQQSQNTN NAFGNQNQLG GSSFGSKPVG 251 SGSLFGQSNN TLGNTTNNRN GLFGQMNSSN QGSSNSGLFG QNSMNSSTQG 301 VFGQNNNQMQ INGNNNNSLF GKANTFSNSA SGGLFGQNNQ QQGSGLFGQN 351 SQTSGSSGLF GQNNQKQPNT FTQSNTGIGL FGQNNNQQQQ STGLFGAKPA 401 GTTGSLFGGN SSTQPNSLFG TTNVPTSNTQ SQQGNSLFGA TKLTNMPFGG 451 NPTANQSGSG NSLFGTKPAS TTGSLFGNNT ASTTVPSTNG LFGNNANNST 501 STTNTGLFGA KPDSQSKPAL GGGLFGNSNS NSSTIGQNKP VFGGTTQNTG 551 LFGATGTNSS AVGSTGKLFG QNNNTLNVGT QNVPPVNNTT QNALLGTTAV 601 PSLQQAPVTN EQLFSKISIP NSITNPVKAT TSKVNADMKR NSSLTSAYRL 651 APKPLFAPSS NGDAKFQKWG KTLERSDRGS STSNSITDPE SSYLNSNDLL 701 FDPDRRYLKH LVIKNNKNLN VINHNDDEAS KVKLVTFTTE SASKDDQASS 751 SIAASKLTEK AHSPQTDLKD DHDESTPDPQ SKSPNGSTSI PMIENEKISS 801 KVPGLLSNDV TFFKNNYYIS PSIETLGNKS LIELRKINNL VIGHRNYGKV 851 EFLEPVDLLN TPLDTLCGDL VTFGPKSCSI YENCSIKPEK GEGINVRCRV 901 TLYSCFPIDK ETRKPIKNIT HPLLKRSIAK LKENPVYKFE SYDPVTGTYS 951 YTIDHPVLT*

Amino Acid Sequence (or in FASTA format)

       1  MFGNNRPMFG GSNLSFGSNT SSFGGQQSQQ PNSLFGNSNN NNNSTSNNAQ
      51  SGFGGFTSAA GSNSNSLFGN NNTQNNGAFG QSMGATQNSP FGSLNSSNAS
     101  NGNTFGGSSS MGSFGGNTNN AFNNNSNSTN SPFGFNKPNT GGTLFGSQNN
     151  NSAGTSSLFG GQSTSTTGTF GNTGSSFGTG LNGNGSNIFG AGNNSQSNTT
     201  GSLFGNQQSS AFGTNNQQGS LFGQQSQNTN NAFGNQNQLG GSSFGSKPVG
     251  SGSLFGQSNN TLGNTTNNRN GLFGQMNSSN QGSSNSGLFG QNSMNSSTQG
     301  VFGQNNNQMQ INGNNNNSLF GKANTFSNSA SGGLFGQNNQ QQGSGLFGQN
     351  SQTSGSSGLF GQNNQKQPNT FTQSNTGIGL FGQNNNQQQQ STGLFGAKPA
     401  GTTGSLFGGN SSTQPNSLFG TTNVPTSNTQ SQQGNSLFGA TKLTNMPFGG
     451  NPTANQSGSG NSLFGTKPAS TTGSLFGNNT ASTTVPSTNG LFGNNANNST
     501  STTNTGLFGA KPDSQSKPAL GGGLFGNSNS NSSTIGQNKP VFGGTTQNTG
     551  LFGATGTNSS AVGSTGKLFG QNNNTLNVGT QNVPPVNNTT QNALLGTTAV
     601  PSLQQAPVTN EQLFSKISIP NSITNPVKAT TSKVNADMKR NSSLTSAYRL
     651  APKPLFAPSS NGDAKFQKWG KTLERSDRGS STSNSITDPE SSYLNSNDLL
     701  FDPDRRYLKH LVIKNNKNLN VINHNDDEAS KVKLVTFTTE SASKDDQASS
     751  SIAASKLTEK AHSPQTDLKD DHDESTPDPQ SKSPNGSTSI PMIENEKISS
     801  KVPGLLSNDV TFFKNNYYIS PSIETLGNKS LIELRKINNL VIGHRNYGKV
     851  EFLEPVDLLN TPLDTLCGDL VTFGPKSCSI YENCSIKPEK GEGINVRCRV
     901  TLYSCFPIDK ETRKPIKNIT HPLLKRSIAK LKENPVYKFE SYDPVTGTYS
     951  YTIDHPVLT*

external links for Nup100p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nup100p

1)	Wente SR, et al. (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
2)	Rout MP, et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
3)	Strawn LA, et al. (2001) The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem 276(9):6445-52
4)	Pyhtila B and Rexach M (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709
5)	Menon BB, et al. (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	Patel SS, et al. (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
8)	Light WH, et al. (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25
9)	Jovanovic-Talisman T, et al. (2009) Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 457(7232):1023-7
10)	Meinema AC, et al. (2011) Long unfolded linkers facilitate membrane protein import through the nuclear pore complex. Science 333(6038):90-3
11)	Halfmann R, et al. (2012) Prion formation by a yeast GLFG nucleoporin. Prion 6(4):391-9
12)	Davis LI and Fink GR (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
13)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41