CSE4/YKL049C Protein Information

Standard Name	Cse4p 1
Systematic Name	Ykl049cp
Alias	Csl2p 2
ORF Classification	Verified
Description	Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions (1, 3, 4, 5, 6)
Name Description	Chromosome SEgregation 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	229
Molecular Weight (Da)	26,841
Isoelectric Point (pI)	10.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cse4p (InterPro)
Physical Interactions	There are 375 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000128518 \| P36012 MIPS: YKL049C NCBI: 1169108 \| 27808712 \| 326327711 \| 429544115 \| 486066 \| 694027 \| NP_012875.2 \| NM_001179615.1 GenBank/EMBL/DDBJ: DAA09108.1 \| U20327 \| X71621 \| Z28049

Amino Acid Sequence (or in FASTA format)
1 MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN 51 LFPRREERRR YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP 101 VRTHSYALDR YVRQKRREKQ RKQSLKRVEK KYTPSELALY EIRKYQRSTD 151 LLISKIPFAR LVKEVTDEFT TKDQDLRWQS MAIMALQEAS EAYLVGLLEH 201 TNLLALHAKR ITIMKKDMQL ARRIRGQFI*

Amino Acid Sequence (or in FASTA format)

       1  MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN
      51  LFPRREERRR YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP
     101  VRTHSYALDR YVRQKRREKQ RKQSLKRVEK KYTPSELALY EIRKYQRSTD
     151  LLISKIPFAR LVKEVTDEFT TKDQDLRWQS MAIMALQEAS EAYLVGLLEH
     201  TNLLALHAKR ITIMKKDMQL ARRIRGQFI*

external links for Cse4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Cse4p

1)	Stoler S, et al. (1995) A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev 9(5):573-86
2)	Baker RE, et al. (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3)	Meluh PB, et al. (1998) Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell 94(5):607-13
4)	Ranjitkar P, et al. (2010) An E3 Ubiquitin Ligase Prevents Ectopic Localization of the Centromeric Histone H3 Variant via the Centromere Targeting Domain. Mol Cell 40(3):455-64
5)	Hewawasam G, et al. (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
6)	Lefrancois P, et al. (2013) Centromere-like regions in the budding yeast genome. PLoS Genet 9(1):e1003209