UGP1/YKL035W Protein Information

Standard Name	Ugp1p 1
Systematic Name	Ykl035wp
ORF Classification	Verified
Description	UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	UDP-glucose pyrophosphorylase 1

Experimental Data
Molecules/cell	17200 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	499
Molecular Weight (Da)	55,987
Isoelectric Point (pI)	7.46

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ugp1p (InterPro)
Physical Interactions	There are 29 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137AC0 \| P32861 MIPS: YKL035W NCBI: 119390051 \| 119390052 \| 398364619 \| 418149 \| 4794 \| 486040 \| NP_012889.3 GenBank/EMBL/DDBJ: DAA09120.1 \| X69584 \| Z28035
External Classifications	EC: 2.7.7.9 [UTP--glucose-1-phosphate uridylyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN 51 ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN 101 LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD 151 SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV 201 PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA 251 TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP 301 KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR 351 DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL 401 FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT 451 ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH*

Amino Acid Sequence (or in FASTA format)

       1  MSTKKHTKTH STYAFESNTN SVAASQMRNA LNKLADSSKL DDAARAKFEN
      51  ELDSFFTLFR RYLVEKSSRT TLEWDKIKSP NPDEVVKYEI ISQQPENVSN
     101  LSKLAVLKLN GGLGTSMGCV GPKSVIEVRE GNTFLDLSVR QIEYLNRQYD
     151  SDVPLLLMNS FNTDKDTEHL IKKYSANRIR IRSFNQSRFP RVYKDSLLPV
     201  PTEYDSPLDA WYPPGHGDLF ESLHVSGELD ALIAQGREIL FVSNGDNLGA
     251  TVDLKILNHM IETGAEYIME LTDKTRADVK GGTLISYDGQ VRLLEVAQVP
     301  KEHIDEFKNI RKFTNFNTNN LWINLKAVKR LIESSNLEME IIPNQKTITR
     351  DGHEINVLQL ETACGAAIRH FDGAHGVVVP RSRFLPVKTC SDLLLVKSDL
     401  FRLEHGSLKL DPSRFGPNPL IKLGSHFKKV SGFNARIPHI PKIVELDHLT
     451  ITGNVFLGKD VTLRGTVIIV CSDGHKIDIP NGSILENVVV TGNLQILEH*

external links for Ugp1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ugp1p

1)	Daran JM, et al. (1995) Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae. Eur J Biochem 233(2):520-30
2)	Daran JM, et al. (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96
3)	Nishizawa M, et al. (2001) Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase. Yeast 18(3):239-49
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41