TCD2/YKL027W Protein Information

Standard Name	Tcd2p 1
Systematic Name	Ykl027wp
ORF Classification	Verified
Description	tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	tRNA ThreonylCarbamoyladenosine Dehydratase 1

Experimental Data
Molecules/cell	1170 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	447
Molecular Weight (Da)	50,265
Isoelectric Point (pI)	9.32

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tcd2p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B6B5 \| P36101 MIPS: YKL027W NCBI: 486025 \| 51012833 \| 549663 \| 6322825 \| NP_012898.1 GenBank/EMBL/DDBJ: DAA09127.1 \| AY692691 \| Z28027

Amino Acid Sequence (or in FASTA format)
1 MVEKDTWKLI TATALFTVAV TTITDYAWTS WQAQKQVIAQ QKNKNKGGQT 51 KSDTDKYHQY DEQFIRQSLK NNVEFLGEDT IEKLSNQYVV VVGAGGVGSW 101 VVNSLVRSGC RKIRVVDFDQ VSLSSLNRHS CAILNDVGTP KVECLRRHMR 151 EIAPWCEIDP INELWTLQNG ERLTLGNGTP DFIVDCIDNI DTKVDLLEFA 201 YNHGIKVISS MGASAKSDPT KLNVGDLATT EEDPLARVVR RKLKKRGILS 251 GIPVVFSAEK PDPKKAKLLP LPDEEYERGK VDELSALKDF RVRILPVLGT 301 MPSLFGLTIT TWILSNISDK PLEPVEGKNR IKVYDGIYQS LAGQMSRVGI 351 PSQRIPLALK DVSYLVEEVF KGKSPISGIS TRLTLTKWDP SKPISLQNVV 401 VLTKNEQKVH EDRVLKGKES LQDVYDAKVL KLVSQRFREE AYYSQFR*

Amino Acid Sequence (or in FASTA format)

       1  MVEKDTWKLI TATALFTVAV TTITDYAWTS WQAQKQVIAQ QKNKNKGGQT
      51  KSDTDKYHQY DEQFIRQSLK NNVEFLGEDT IEKLSNQYVV VVGAGGVGSW
     101  VVNSLVRSGC RKIRVVDFDQ VSLSSLNRHS CAILNDVGTP KVECLRRHMR
     151  EIAPWCEIDP INELWTLQNG ERLTLGNGTP DFIVDCIDNI DTKVDLLEFA
     201  YNHGIKVISS MGASAKSDPT KLNVGDLATT EEDPLARVVR RKLKKRGILS
     251  GIPVVFSAEK PDPKKAKLLP LPDEEYERGK VDELSALKDF RVRILPVLGT
     301  MPSLFGLTIT TWILSNISDK PLEPVEGKNR IKVYDGIYQS LAGQMSRVGI
     351  PSQRIPLALK DVSYLVEEVF KGKSPISGIS TRLTLTKWDP SKPISLQNVV
     401  VLTKNEQKVH EDRVLKGKES LQDVYDAKVL KLVSQRFREE AYYSQFR*

external links for Tcd2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Tcd2p

1)	Miyauchi K, et al. (2013) A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification. Nat Chem Biol 9(2):105-11
2)	Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Zahedi RP, et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41