SWD2/YKL018W Protein Information

Standard Name	Swd2p 1
Systematic Name	Ykl018wp
Alias	Cps35p 2 , Saf37p 3
ORF Classification	Verified
Description	Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination (1, 2, 3, 4, 5, 6, 7)
Name Description	Set1c, WD40 repeat protein

Experimental Data
Molecules/cell	704 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	329
Molecular Weight (Da)	37,122
Isoelectric Point (pI)	5.66

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Swd2p (InterPro)
Physical Interactions	There are 141 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DD0 \| P36104 MIPS: YKL018W NCBI: 398364727 \| 45270078 \| 486009 \| 549660 \| NP_012907.3 GenBank/EMBL/DDBJ: DAA09138.1 \| AY558094 \| X74152 \| Z28018

Amino Acid Sequence (or in FASTA format)
1 MTTVSINKPN LLKFKHVKSF QPQEKDCGPV TSLNFDDNGQ FLLTSSSNDT 51 MQLYSATNCK FLDTIASKKY GCHSAIFTHA QNECIYSSTM KNFDIKYLNL 101 ETNQYLRYFS GHGALVNDLK MNPVNDTFLS SSYDESVRLW DLKISKPQVI 151 IPSLVPNCIA YDPSGLVFAL GNPENFEIGL YNLKKIQEGP FLIIKINDAT 201 FSQWNKLEFS NNGKYLLVGS SIGKHLIFDA FTGQQLFELI GTRAFPMREF 251 LDSGSACFTP DGEFVLGTDY DGRIAIWNHS DSISNKVLRP QGFIPCVSHE 301 TCPRSIAFNP KYSMFVTADE TVDFYVYDE*

Amino Acid Sequence (or in FASTA format)

       1  MTTVSINKPN LLKFKHVKSF QPQEKDCGPV TSLNFDDNGQ FLLTSSSNDT
      51  MQLYSATNCK FLDTIASKKY GCHSAIFTHA QNECIYSSTM KNFDIKYLNL
     101  ETNQYLRYFS GHGALVNDLK MNPVNDTFLS SSYDESVRLW DLKISKPQVI
     151  IPSLVPNCIA YDPSGLVFAL GNPENFEIGL YNLKKIQEGP FLIIKINDAT
     201  FSQWNKLEFS NNGKYLLVGS SIGKHLIFDA FTGQQLFELI GTRAFPMREF
     251  LDSGSACFTP DGEFVLGTDY DGRIAIWNHS DSISNKVLRP QGFIPCVSHE
     301  TCPRSIAFNP KYSMFVTADE TVDFYVYDE*

external links for Swd2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Swd2p

1)	Roguev A, et al. (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J 20(24):7137-48
2)	Miller T, et al. (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci U S A 98(23):12902-7
3)	Nagy PL, et al. (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99(1):90-4
4)	Krogan NJ, et al. (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277(13):10753-5
5)	Sun ZW and Allis CD (2002) Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast. Nature 418(6893):104-8
6)	Dover J, et al. (2002) Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem 277(32):28368-71
7)	Cheng H, et al. (2004) The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3. Mol Cell Biol 24(7):2932-43
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41