HCS1/YKL017C Protein Information Help

Standard Name Hcs1p 1
Systematic Name Ykl017cp
Alias Dip1p
ORF Classification Verified
Description Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities (2, 3, 4)
Name Description dna HeliCaSe 3
Experimental Data
Molecules/cell 1040 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 683
Molecular Weight (Da) 78,272
Isoelectric Point (pI) 7.56

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hcs1p (InterPro)
Physical Interactions There are 1 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B6B1 | P34243
MIPS: YKL017C
NCBI: 395256 | 465704 | 486007 | 6322835 | NP_012908.1 | NM_001179583.1
GenBank/EMBL/DDBJ: CAA52266.1 | CAA81852.1 | DAA09139.1 | X74152 | Z28017
External Classifications EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
       1  MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN
      51  IRSGLIGKLY MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK
     101  VKKVSEDSNG EQAECSGVVY KMSDTQITIA LEESQDVIAT TFYSYSKLYI
     151  LKTTNVVTYN RMESTMRKLS EISSPIQDKI IQYLVNERPF IPNTNSFQNI
     201  KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI ELIQQLLIKN
     251  PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD
     301  ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE
     351  FKTIKDLIIQ SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA
     401  MEPQCWIPLI AHQNQFHKLV LAGDNKQLPP TIKTEDDKNV IHNLETTLFD
     451  RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS HSMYNGKLLA DATVANRLLI
     501  DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL GSKYNEGEIA
     551  IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV
     601  DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI
     651  EVLQRCGNKY LKSWSEWCEE NADVRYPNID DYL*                 

external links for Hcs1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) CYC2008 (complexes) MIPS YeastRC Public Image Repository
YGOB Complexome Pfam domains
YOGY DIP YeastRC Structure Prediction (Seattle)

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Hcs1p
1) Shiratori A, et al.  (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53
2) Biswas SB, et al.  (1997) Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A. Biochemistry 36(43):13270-6
3) Biswas EE, et al.  (1997) Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization. Biochemistry 36(43):13277-84
4) Biswas EE, et al.  (1993) DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography. Biochemistry 32(49):13393-8
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41