HCS1/YKL017C Protein Information

Standard Name	Hcs1p
Systematic Name	Ykl017cp
Alias	Dip1p
ORF Classification	Verified
Description	Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities (1, 2, 3)
Name Description	dna HeliCaSe 2

Experimental Data
Molecules/cell	1040 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	683
Molecular Weight (Da)	78,272
Isoelectric Point (pI)	7.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hcs1p (InterPro)
Physical Interactions	There are 1 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B6B1 \| P34243 MIPS: YKL017C NCBI: 395256 \| 465704 \| 486007 \| 6322835 \| NP_012908.1 \| NM_001179583.1 GenBank/EMBL/DDBJ: DAA09139.1 \| X74152 \| Z28017
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN 51 IRSGLIGKLY MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK 101 VKKVSEDSNG EQAECSGVVY KMSDTQITIA LEESQDVIAT TFYSYSKLYI 151 LKTTNVVTYN RMESTMRKLS EISSPIQDKI IQYLVNERPF IPNTNSFQNI 201 KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI ELIQQLLIKN 251 PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD 301 ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE 351 FKTIKDLIIQ SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA 401 MEPQCWIPLI AHQNQFHKLV LAGDNKQLPP TIKTEDDKNV IHNLETTLFD 451 RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS HSMYNGKLLA DATVANRLLI 501 DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL GSKYNEGEIA 551 IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV 601 DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI 651 EVLQRCGNKY LKSWSEWCEE NADVRYPNID DYL*

Amino Acid Sequence (or in FASTA format)

       1  MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN
      51  IRSGLIGKLY MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK
     101  VKKVSEDSNG EQAECSGVVY KMSDTQITIA LEESQDVIAT TFYSYSKLYI
     151  LKTTNVVTYN RMESTMRKLS EISSPIQDKI IQYLVNERPF IPNTNSFQNI
     201  KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI ELIQQLLIKN
     251  PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD
     301  ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE
     351  FKTIKDLIIQ SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA
     401  MEPQCWIPLI AHQNQFHKLV LAGDNKQLPP TIKTEDDKNV IHNLETTLFD
     451  RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS HSMYNGKLLA DATVANRLLI
     501  DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL GSKYNEGEIA
     551  IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV
     601  DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI
     651  EVLQRCGNKY LKSWSEWCEE NADVRYPNID DYL*

external links for Hcs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hcs1p

1)	Biswas SB, et al. (1997) Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A. Biochemistry 36(43):13270-6
2)	Biswas EE, et al. (1997) Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization. Biochemistry 36(43):13277-84
3)	Biswas EE, et al. (1993) DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography. Biochemistry 32(49):13393-8
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41