PUT3/YKL015W Protein Information

Standard Name	Put3p
Systematic Name	Ykl015wp
ORF Classification	Verified
Description	Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain (1, 2, 3, 4, 5)
Name Description	Proline UTilization

Experimental Data
Molecules/cell	736 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	979
Molecular Weight (Da)	111,413
Isoelectric Point (pI)	5.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Put3p (InterPro)
Transmembrane Domains	There are 2 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000132B7F \| P25502 MIPS: YKL015W NCBI: 131653 \| 157838150 \| 157838151 \| 3660288 \| 3660289 \| 395257 \| 4252 \| 486003 \| 6322837 \| NP_012910.1 GenBank/EMBL/DDBJ: DAA09141.1 \| X55384 \| X74152 \| Z28015

Amino Acid Sequence (or in FASTA format)
1 MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC 51 QKCVTSNAIC EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER 101 PVSVRGKDNS DDDERHINNA PSSDTLEVSS APAAPIFDLM SNSNTASDND 151 NDDDNSNRIT NNRSYDHSLE KYYKKAISIF KQPANANGEN GNGANGHEDD 201 DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE IQSLVTKYIS 251 VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL 301 NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY 351 NDGCFYFFNE GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC 401 KILLILAVGE MYLGSINNEM LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE 451 RLENVTKKGG IEVLLLYAFF LQVADYTLAS YFYFGQALRT CLILGLHVDS 501 QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY TISTALPADI 551 DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL 601 KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV 651 RPLLFHFASI QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW 701 SLLQNSMLAM FSYMDREYLF TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF 751 TQMRNLGNIP AGLRRAQLLT LMANLDFHGI MNDLITKYND ILKFDSMNCE 801 NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN TNMIKNESIS 851 NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ 901 TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI 951 TDQAMWLGNT MDPTAAAGSE IDFTDYLGP*

Amino Acid Sequence (or in FASTA format)

       1  MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC
      51  QKCVTSNAIC EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER
     101  PVSVRGKDNS DDDERHINNA PSSDTLEVSS APAAPIFDLM SNSNTASDND
     151  NDDDNSNRIT NNRSYDHSLE KYYKKAISIF KQPANANGEN GNGANGHEDD
     201  DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE IQSLVTKYIS
     251  VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL
     301  NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY
     351  NDGCFYFFNE GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC
     401  KILLILAVGE MYLGSINNEM LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE
     451  RLENVTKKGG IEVLLLYAFF LQVADYTLAS YFYFGQALRT CLILGLHVDS
     501  QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY TISTALPADI
     551  DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL
     601  KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV
     651  RPLLFHFASI QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW
     701  SLLQNSMLAM FSYMDREYLF TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF
     751  TQMRNLGNIP AGLRRAQLLT LMANLDFHGI MNDLITKYND ILKFDSMNCE
     801  NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN TNMIKNESIS
     851  NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ
     901  TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI
     951  TDQAMWLGNT MDPTAAAGSE IDFTDYLGP*

external links for Put3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Put3p

1)	Siddiqui AH and Brandriss MC (1989) The Saccharomyces cerevisiae PUT3 activator protein associates with proline-specific upstream activation sequences. Mol Cell Biol 9(11):4706-12
2)	Huang HL and Brandriss MC (2000) The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source. Mol Cell Biol 20(3):892-9
3)	des Etages SA, et al. (1996) Functional analysis of the PUT3 transcriptional activator of the proline utilization pathway in Saccharomyces cerevisiae. Genetics 142(4):1069-82
4)	Brandriss MC (1987) Evidence for positive regulation of the proline utilization pathway in Saccharomyces cerevisiae. Genetics 117(3):429-35
5)	Des Etages SA, et al. (2001) Conformational changes play a role in regulating the activity of the proline utilization pathway-specific regulator in Saccharomyces cerevisiae. Mol Microbiol 40(4):890-9
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41