UFD4/YKL010C Protein Information

Standard Name	Ufd4p 1
Systematic Name	Ykl010cp
ORF Classification	Verified
Description	Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress (2, 3, 4, 5)
Name Description	Ubiquitin Fusion Degradation protein 1

Experimental Data
Molecules/cell	7380 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,483
Molecular Weight (Da)	167,841
Isoelectric Point (pI)	4.62

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ufd4p (InterPro)
Physical Interactions	There are 49 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000137AD6 \| P33202 MIPS: YKL010C NCBI: 263499 \| 398364789 \| 465668 \| 485993 \| NP_012915.3 GenBank/EMBL/DDBJ: DAA09146.1 \| S53418 \| Z28010

Amino Acid Sequence (or in FASTA format)
1 MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED 51 EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED 101 GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE 151 ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI 201 LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV 251 CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK 301 TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP 351 IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ 401 DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN 451 AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN 501 QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI 551 NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL 601 FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC 651 DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL 701 SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH 751 EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE 801 ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD 851 LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE 901 ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH 951 TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL 1001 LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP 1051 FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR 1101 RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV 1151 SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV 1201 IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET 1251 CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG 1301 CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF 1351 PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH 1401 ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC 1451 ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS*

Amino Acid Sequence (or in FASTA format)

       1  MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED
      51  EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED
     101  GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE
     151  ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI
     201  LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV
     251  CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK
     301  TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP
     351  IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ
     401  DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN
     451  AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN
     501  QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI
     551  NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL
     601  FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC
     651  DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL
     701  SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH
     751  EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE
     801  ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD
     851  LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE
     901  ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH
     951  TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL
    1001  LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP
    1051  FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR
    1101  RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV
    1151  SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV
    1201  IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET
    1251  CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG
    1301  CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF
    1351  PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH
    1401  ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC
    1451  ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS*

external links for Ufd4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ufd4p

1)	Johnson ES, et al. (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270(29):17442-56
2)	Xie Y and Varshavsky A (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502
3)	Hochstrasser M (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39
4)	Xie Y and Varshavsky A (2002) UFD4 lacking the proteasome-binding region catalyses ubiquitination but is impaired in proteolysis. Nat Cell Biol 4(12):1003-7
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41