LYS1/YIR034C Protein Information

Standard Name	Lys1p
Systematic Name	Yir034cp
ORF Classification	Verified
Description	Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity (1, 2)
Name Description	LYSine requiring

Experimental Data
Molecules/cell	112000 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	373
Molecular Weight (Da)	41,464
Isoelectric Point (pI)	9.82

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Lys1p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168354 \| P38998 MIPS: YIR034C NCBI: 159795344 \| 159795345 \| 159795346 \| 160285837 \| 374414637 \| 374414638 \| 374414639 \| 374414640 \| 398364729 \| 453184 \| 557840 \| 82654956 \| NP_012300.3 GenBank/EMBL/DDBJ: DAA08581.1 \| X77362 \| Z38061
External Classifications	EC: 1.5.1.7 [Saccharopine dehydrogenase (NAD(+), L-lysine-forming)]

Amino Acid Sequence (or in FASTA format)
1 MAAVTLHLRA ETKPLEARAA LTPTTVKKLI AKGFKIYVED SPQSTFNINE 51 YRQAGAIIVP AGSWKTAPRD RIIIGLKEMP ETDTFPLVHE HIQFAHCYKD 101 QAGWQNVLMR FIKGHGTLYD LEFLENDQGR RVAAFGFYAG FAGAALGVRD 151 WAFKQTHSDD EDLPAVSPYP NEKALVKDVT KDYKEALATG ARKPTVLIIG 201 ALGRCGSGAI DLLHKVGIPD ANILKWDIKE TSRGGPFDEI PQADIFINCI 251 YLSKPIAPFT NMEKLNNPNR RLRTVVDVSA DTTNPHNPIP IYTVATVFNK 301 PTVLVPTTAG PKLSVISIDH LPSLLPREAS EFFSHDLLPS LELLPQRKTA 351 PVWVRAKKLF DRHCARVKRS SRL*

Amino Acid Sequence (or in FASTA format)

       1  MAAVTLHLRA ETKPLEARAA LTPTTVKKLI AKGFKIYVED SPQSTFNINE
      51  YRQAGAIIVP AGSWKTAPRD RIIIGLKEMP ETDTFPLVHE HIQFAHCYKD
     101  QAGWQNVLMR FIKGHGTLYD LEFLENDQGR RVAAFGFYAG FAGAALGVRD
     151  WAFKQTHSDD EDLPAVSPYP NEKALVKDVT KDYKEALATG ARKPTVLIIG
     201  ALGRCGSGAI DLLHKVGIPD ANILKWDIKE TSRGGPFDEI PQADIFINCI
     251  YLSKPIAPFT NMEKLNNPNR RLRTVVDVSA DTTNPHNPIP IYTVATVFNK
     301  PTVLVPTTAG PKLSVISIDH LPSLLPREAS EFFSHDLLPS LELLPQRKTA
     351  PVWVRAKKLF DRHCARVKRS SRL*

external links for Lys1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Lys1p

1)	Ogawa H and Fujioka M (1978) Purification and characterization of saccharopine dehydrogenase from baker's yeast. J Biol Chem 253(10):3666-70
2)	Tsvetanova NG, et al. (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41