MGA2/YIR033W Protein Information

Standard Name	Mga2p
Systematic Name	Yir033wp
ORF Classification	Verified
Description	ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication (1, 2, 3)
Name Description	Multicopy suppressor of GAm1 (snf2) 4

Experimental Data
Molecules/cell	300 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,113
Molecular Weight (Da)	127,053
Isoelectric Point (pI)	4.89

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mga2p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 41 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053083 \| P40578 MIPS: YIR033W NCBI: 557839 \| 6322224 \| 731921 \| NP_012299.1 GenBank/EMBL/DDBJ: DAA08580.1 \| Z38061

Amino Acid Sequence (or in FASTA format)
1 MQQNSEFLTE TPGSDPHISQ LHANSVMESQ LLDDFLLNGS PMYQDDSMAH 51 INIDEGANFQ NFIKTDEGDS PNLLSFEGIG NNTHVNQNVS TPLEEEMESN 101 RALKEEEEDE HENKVFNEKN IGNPAHDEIV FGRKETIQSV YINPLDYLKV 151 NAAQLPLDVE VSGLPQVSRV ENQLKLKVKI TSETPLNQSM LYLPSDSISR 201 EKFYLKKNIE DFSEDFKKNL LYINAFVLCA VSNRTTNVCT KCVKREQRRA 251 ARRKSGIADN LLWCNNINRR LVVFNNKQVF PIMKTFDNVK EFELTTRLVC 301 YCRHHKANNG FVILFTITDW QNRLLGKFTT TPIMITDRKP ANMDTTKFNN 351 TTTSSRRQLT EEESTTEYYS TDNNQLSKDE NMPFQYTYQH NPYDNDSQMN 401 NIPLKDKNVP FPYSISQQTD LLQNNNLSLN LSLPNQHIPS PTSMSEEGSE 451 SFNYHHRDND NPVRTISLTN IEQQSQLNQR KRARNNLEND IGKPLFKHSF 501 SNSISATNTM NPALHSMQDF SMKNNNNNLP SINRVIPSQG PINGGIEVTL 551 LGCNFKDGLS VKFGSNLALS TQCWSETTIV TYLPPAAYAG QVFVSITDTN 601 NENNNDDLPQ EIEINDNKKA IFTYVDDTDR QLIELALQIV GLKMNGKLED 651 ARNIAKRIVG NDSPDSGTNG NSCSKSTGPS PNQHSMNLNT SVLYSDEVLI 701 QKVIKSLNIN SNISICDSLG RTLLHLACLK NYSSLVYTLI KKGARVNDID 751 SFGLTPLHFA CISGDPKIIK MLLNCKVNYS LRSHNGLTAR EVFIANHIHS 801 KEIDKKQDNR DNHKFVHNDT YISEVLSLFE EFQNGTKFTD SVETDSNYSI 851 SRKYSQSSFN SSLLDNESLN ENLFESQSMI NPTSMEIQHP TLQLFENSSY 901 SEYDQSDFEE DGDEDLFVTD EVEKPGVACR EEQSELLDIG SSANEPEEDN 951 GSTSLWNRVL HRINDDLPKY EDLFPLSWGK DDKLKTTNQD SIVEQSASNI 1001 ENSENSEEED YEEEEEFLKK QFNRFFQNKQ NFRNDKMLIF FWIPLTLLLL 1051 TWFIMYKFGN QDSSINHISE LISEYLRIAL AKFLLGNERM KTAFRSKLSN 1101 LQTTRMLNDL IVS*

Amino Acid Sequence (or in FASTA format)

       1  MQQNSEFLTE TPGSDPHISQ LHANSVMESQ LLDDFLLNGS PMYQDDSMAH
      51  INIDEGANFQ NFIKTDEGDS PNLLSFEGIG NNTHVNQNVS TPLEEEMESN
     101  RALKEEEEDE HENKVFNEKN IGNPAHDEIV FGRKETIQSV YINPLDYLKV
     151  NAAQLPLDVE VSGLPQVSRV ENQLKLKVKI TSETPLNQSM LYLPSDSISR
     201  EKFYLKKNIE DFSEDFKKNL LYINAFVLCA VSNRTTNVCT KCVKREQRRA
     251  ARRKSGIADN LLWCNNINRR LVVFNNKQVF PIMKTFDNVK EFELTTRLVC
     301  YCRHHKANNG FVILFTITDW QNRLLGKFTT TPIMITDRKP ANMDTTKFNN
     351  TTTSSRRQLT EEESTTEYYS TDNNQLSKDE NMPFQYTYQH NPYDNDSQMN
     401  NIPLKDKNVP FPYSISQQTD LLQNNNLSLN LSLPNQHIPS PTSMSEEGSE
     451  SFNYHHRDND NPVRTISLTN IEQQSQLNQR KRARNNLEND IGKPLFKHSF
     501  SNSISATNTM NPALHSMQDF SMKNNNNNLP SINRVIPSQG PINGGIEVTL
     551  LGCNFKDGLS VKFGSNLALS TQCWSETTIV TYLPPAAYAG QVFVSITDTN
     601  NENNNDDLPQ EIEINDNKKA IFTYVDDTDR QLIELALQIV GLKMNGKLED
     651  ARNIAKRIVG NDSPDSGTNG NSCSKSTGPS PNQHSMNLNT SVLYSDEVLI
     701  QKVIKSLNIN SNISICDSLG RTLLHLACLK NYSSLVYTLI KKGARVNDID
     751  SFGLTPLHFA CISGDPKIIK MLLNCKVNYS LRSHNGLTAR EVFIANHIHS
     801  KEIDKKQDNR DNHKFVHNDT YISEVLSLFE EFQNGTKFTD SVETDSNYSI
     851  SRKYSQSSFN SSLLDNESLN ENLFESQSMI NPTSMEIQHP TLQLFENSSY
     901  SEYDQSDFEE DGDEDLFVTD EVEKPGVACR EEQSELLDIG SSANEPEEDN
     951  GSTSLWNRVL HRINDDLPKY EDLFPLSWGK DDKLKTTNQD SIVEQSASNI
    1001  ENSENSEEED YEEEEEFLKK QFNRFFQNKQ NFRNDKMLIF FWIPLTLLLL
    1051  TWFIMYKFGN QDSSINHISE LISEYLRIAL AKFLLGNERM KTAFRSKLSN
    1101  LQTTRMLNDL IVS*

external links for Mga2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mga2p

1)	Chellappa R, et al. (2001) The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem 276(47):43548-56
2)	Shcherbik N, et al. (2003) Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90. Curr Biol 13(14):1227-33
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Zhang S, et al. (1997) Genetic redundancy between SPT23 and MGA2: regulators of Ty-induced mutations and Ty1 transcription in Saccharomyces cerevisiae. Mol Cell Biol 17(8):4718-29
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41