MRS1/YIR021W Protein Information

Standard Name	Mrs1p 1
Systematic Name	Yir021wp
Alias	Pet157p
ORF Classification	Verified
Description	Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Mitochondrial RNA Splicing 1

Experimental Data
Molecules/cell	5240 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	363
Molecular Weight (Da)	41,289
Isoelectric Point (pI)	8.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mrs1p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012F58B \| P07266 MIPS: YIR021W NCBI: 398364657 \| 3988 \| 557827 \| 730063 \| NP_012287.3 GenBank/EMBL/DDBJ: DAA08567.1 \| X05509 \| Z38061

Amino Acid Sequence (or in FASTA format)
1 MSPKNITRSV IPAIDLYCRK ANFKTLKSLS MILGSKKEWY DTKKAPLRTF 51 LVSRCGIFEQ LRGRLVEDGK VNLFSVFLTN DSFSFCKMTV DDKFNTSLVD 101 WQKIPFDSTF ATDRRQNISL LPVDTLFATE KIISILGVSP NMTNLVSIER 151 ERSDLVDFNC KLQSNILEHL LYAKCQGVYV TSTNEKARLL AAVCNPEFID 201 TFWCELTPIR VSLKENPSIS VPREYQMYDP VVRATIKEVV TKRLLRSAFD 251 NDIDPLMCLH LDKGWKLKFP ILSSTTGLNF SLKDCLSLDT GKDASDMTEV 301 FLATMESSKV LRTYSNLVDI VMKDNGRLDS GVLKQFNDYV KQEKLNLQHF 351 QAGSSKFLKG AKI*

Amino Acid Sequence (or in FASTA format)

       1  MSPKNITRSV IPAIDLYCRK ANFKTLKSLS MILGSKKEWY DTKKAPLRTF
      51  LVSRCGIFEQ LRGRLVEDGK VNLFSVFLTN DSFSFCKMTV DDKFNTSLVD
     101  WQKIPFDSTF ATDRRQNISL LPVDTLFATE KIISILGVSP NMTNLVSIER
     151  ERSDLVDFNC KLQSNILEHL LYAKCQGVYV TSTNEKARLL AAVCNPEFID
     201  TFWCELTPIR VSLKENPSIS VPREYQMYDP VVRATIKEVV TKRLLRSAFD
     251  NDIDPLMCLH LDKGWKLKFP ILSSTTGLNF SLKDCLSLDT GKDASDMTEV
     301  FLATMESSKV LRTYSNLVDI VMKDNGRLDS GVLKQFNDYV KQEKLNLQHF
     351  QAGSSKFLKG AKI*

external links for Mrs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mrs1p

1)	Kreike J, et al. (1986) Cloning of a nuclear gene MRS1 involved in the excision of a single group I intron (bI3) from the mitochondrial COB transcript in S. cerevisiae. Curr Genet 11(3):185-91
2)	Bousquet I, et al. (1990) Two group I mitochondrial introns in the cob-box and coxI genes require the same MRS1/PET157 nuclear gene product for splicing. Curr Genet 18(2):117-24
3)	Bassi GS, et al. (2002) Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA. Proc Natl Acad Sci U S A 99(1):128-33
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41