SQT1/YIR012W Protein Information

Standard Name	Sqt1p
Systematic Name	Yir012wp
ORF Classification	Verified
Description	Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress (1, 2)

Experimental Data
Molecules/cell	19700 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	431
Molecular Weight (Da)	47,167
Isoelectric Point (pI)	4.18

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sqt1p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135EA6 \| P35184 MIPS: YIR012W NCBI: 2231310 \| 415402 \| 556858 \| 6322202 \| 731912 \| NP_012277.1 GenBank/EMBL/DDBJ: DAA08558.1 \| U75717 \| X75916 \| X79743 \| Z37996

Amino Acid Sequence (or in FASTA format)
1 MEPQEEFITT EEVEQEIVPT VEVEQDVPVD IEGENDDDDE MMNDDEEALE 51 VDMSNNSLTY FDKHTDSVFA IGHHPNLPLV CTGGGDNLAH LWTSHSQPPK 101 FAGTLTGYGE SVISCSFTSE GGFLVTADMS GKVLVHMGQK GGAQWKLASQ 151 MQEVEEIVWL KTHPTIARTF AFGATDGSVW CYQINEQDGS LEQLMSGFVH 201 QQDCSMGEFI NTDKGENTLE LVTCSLDSTI VAWNCFTGQQ LFKITQAEIK 251 GLEAPWISLS LAPETLTKGN SGVVACGSNN GLLAVINCNN GGAILHLSTV 301 IELKPEQDEL DASIESISWS SKFSLMAIGL VCGEILLYDT SAWRVRHKFV 351 LEDSVTKLMF DNDDLFASCI NGKVYQFNAR TGQEKFVCVG HNMGVLDFIL 401 LHPVANTGTE QKRKVITAGD EGVSLVFEVP N*

Amino Acid Sequence (or in FASTA format)

       1  MEPQEEFITT EEVEQEIVPT VEVEQDVPVD IEGENDDDDE MMNDDEEALE
      51  VDMSNNSLTY FDKHTDSVFA IGHHPNLPLV CTGGGDNLAH LWTSHSQPPK
     101  FAGTLTGYGE SVISCSFTSE GGFLVTADMS GKVLVHMGQK GGAQWKLASQ
     151  MQEVEEIVWL KTHPTIARTF AFGATDGSVW CYQINEQDGS LEQLMSGFVH
     201  QQDCSMGEFI NTDKGENTLE LVTCSLDSTI VAWNCFTGQQ LFKITQAEIK
     251  GLEAPWISLS LAPETLTKGN SGVVACGSNN GLLAVINCNN GGAILHLSTV
     301  IELKPEQDEL DASIESISWS SKFSLMAIGL VCGEILLYDT SAWRVRHKFV
     351  LEDSVTKLMF DNDDLFASCI NGKVYQFNAR TGQEKFVCVG HNMGVLDFIL
     401  LHPVANTGTE QKRKVITAGD EGVSLVFEVP N*

external links for Sqt1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sqt1p

1)	Eisinger DP, et al. (1997) SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1. Mol Cell Biol 17(9):5146-55
2)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41