MPH1/YIR002C Protein Information

Standard Name	Mph1p 1
Systematic Name	Yir002cp
ORF Classification	Verified
Description	3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases (2, 3, 4, 5, 6, 7, 8)
Name Description	Mutator PHenotype 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	993
Molecular Weight (Da)	114,057
Isoelectric Point (pI)	7.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mph1p (InterPro)
Physical Interactions	There are 29 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005304E \| P40562 MIPS: YIR002C NCBI: 557851 \| 6322192 \| 731905 \| NP_012267.1 GenBank/EMBL/DDBJ: DAA08548.1 \| X79743 \| Z38062
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG 51 QKTVYEEIQR DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF 101 QNTLCAIPTG MGKTFIASTV MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC 151 LGITGIPSDQ TAILLDKSRK NREEIWANKR VFFATPQVVE NDLKRGVLDP 201 KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT ATPASDLEGV 251 QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG 301 MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN 351 FFILQLLNNV GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA 401 AEFYYHPILK NIKNQCENYL SDPKFVGHGK LQCVRDELMD FFQKRGSDSR 451 VIIFTELRES ALEIVKFIDS VADDQIRPHI FIGQARAKEG FDEVKYTRKH 501 APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT GSSEEAQISG 551 MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK 601 NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK 651 KSDRIIPEDI IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV 701 KPKKAITKKK RVQENKKPKK FFMPDNVETS IVSASTLINK FLVNESGGKQ 751 LVTSNENPSK KRKIFKALDN LENDSTEEAS SSLETEDEEV SDDNNVFIAE 801 GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND CGLPTKIEKN 851 VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN 901 FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD 951 EKKFVKVNDK SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF*

Amino Acid Sequence (or in FASTA format)

       1  MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG
      51  QKTVYEEIQR DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF
     101  QNTLCAIPTG MGKTFIASTV MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC
     151  LGITGIPSDQ TAILLDKSRK NREEIWANKR VFFATPQVVE NDLKRGVLDP
     201  KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT ATPASDLEGV
     251  QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG
     301  MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN
     351  FFILQLLNNV GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA
     401  AEFYYHPILK NIKNQCENYL SDPKFVGHGK LQCVRDELMD FFQKRGSDSR
     451  VIIFTELRES ALEIVKFIDS VADDQIRPHI FIGQARAKEG FDEVKYTRKH
     501  APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT GSSEEAQISG
     551  MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK
     601  NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK
     651  KSDRIIPEDI IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV
     701  KPKKAITKKK RVQENKKPKK FFMPDNVETS IVSASTLINK FLVNESGGKQ
     751  LVTSNENPSK KRKIFKALDN LENDSTEEAS SSLETEDEEV SDDNNVFIAE
     801  GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND CGLPTKIEKN
     851  VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN
     901  FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD
     951  EKKFVKVNDK SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF*

external links for Mph1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Mph1p

1)	Entian KD, et al. (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2)	Shiratori A, et al. (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53
3)	Scheller J, et al. (2000) MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage. Genetics 155(3):1069-81
4)	Schurer KA, et al. (2004) Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics 166(4):1673-86
5)	Meetei AR, et al. (2005) A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M. Nat Genet 37(9):958-63
6)	Kang YH, et al. (2009) The MPH1 Gene of Saccharomyces cerevisiae Functions in Okazaki Fragment Processing. J Biol Chem 284(16):10376-86
7)	Yan Z, et al. (2010) A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability. Mol Cell 37(6):865-78
8)	Ward TA, et al. (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884