TID3/YIL144W Protein Information Help

Standard Name Tid3p
Systematic Name Yil144wp
Alias Ndc80p , Hec1p
ORF Classification Verified
Description Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering (1, 2, 3, 4, 5, 6)
Name Description Two-hybrid Interaction with Dmc1p 7
Experimental Data
Molecules/cell 1160 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 691
Molecular Weight (Da) 80,487
Isoelectric Point (pI) 9.39

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tid3p (InterPro)
Physical Interactions There are 170 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B451 | P40460
MIPS: YIL144W
NCBI: 398364235 | 557779 | 731880 | NP_012122.3 | NM_001179492.3
GenBank/EMBL/DDBJ: DAA08409.1 | Z38059
Amino Acid Sequence (or in FASTA format)
 
       1  MQSSTSTDQH VLHHMDPHRF TSQIPTATSS QLRRRNSTNQ GLTDMINKSI
      51  ARNTISGTGI PTGGINKNKR TRSTVAGGTN GTALALNDKS NSRNSVSRLS
     101  INQLGSLQQH LSNRDPRPLR DKNFQSAIQE EIYDYLKKNK FDIETNHPIS
     151  IKFLKQPTQK GFIIIFKWLY LRLDPGYGFT KSIENEIYQI LKNLRYPFLE
     201  SINKSQISAV GGSNWHKFLG MLHWMVRTNI KLDMCLNKVD RSLINQNTQE
     251  ITILSQPLKT LDEQDQRQER YELMVEKLLI DYFTESYKSF LKLEDNYEPS
     301  MQELKLGFEK FVHIINTDIA NLQTQNDNLY EKYQEVMKIS QKIKTTREKW
     351  KALKSDSNKY ENYVNAMKQK SQEWPGKLEK MKSECELKEE EIKALQSNIS
     401  ELHKILRKKG ISTEQFELQN QEREKLTREL DKINIQSDKL TSSIKSRKLE
     451  AEGIFKSLLD TLRQYDSSIQ NLTRSRSQLG HNVNDSSLKI NISENLLDRD
     501  FHEGISYEQL FPKGSGINES IKKSILKLND EIQERIKTIE KDNITLEKDI
     551  KNLKHDINEK TQINEKLELE LSEANSKFEL SKQENERLLV AQRIEIEKME
     601  KKINDSNLLM KTKISDAEEL VTSTELKLEE LKVDLNRKRY KLHQQVIHVI
     651  DITSKFKINI QSSLENSENE LGNVIEELRN LEFETEHNVT N*

external links for Tid3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
YGOB CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Tid3p
1) Zheng L, et al.  (1999) Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol 19(8):5417-28
2) Wigge PA and Kilmartin JV  (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
3) Janke C, et al.  (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
4) McCleland ML, et al.  (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1):101-14
5) Westermann S, et al.  (2003) Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol 163(2):215-22
6) He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206
7) Dresser ME, et al.  (1997) DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics 147(2):533-44
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41