RPI1/YIL119C Protein Information

Standard Name	Rpi1p 1
Systematic Name	Yil119cp
ORF Classification	Verified
Description	Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF (1, 2, 3, 4)
Name Description	Ras-cAMP Pathway Inhibitor 1

Experimental Data
Molecules/cell	1340 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	407
Molecular Weight (Da)	46,622
Isoelectric Point (pI)	9.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpi1p (InterPro)
Physical Interactions	There are 5 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000134633 \| P23250 MIPS: YIL119C NCBI: 172480 \| 233044 \| 398364259 \| 45270066 \| 599998 \| 730607 \| NP_012147.3 GenBank/EMBL/DDBJ: DAA08434.1 \| AY558088 \| M63178 \| S42682 \| Z46833

Amino Acid Sequence (or in FASTA format)
1 MYLEYLQPKL NLMDESSTIS KNFPDYSPNL NTPITSNFNE ETGSDCSLVT 51 PRIISSSNSN SNSNSNSNSN SNSGSIDENE LNNSNSSSSS ARQIRKKWKE 101 PEDIAFITTI MNNSQLLTFV EYFKPMKNFW KKISKILFQQ YGYERNSRQC 151 HDRFKVLYTK SLKVHPSKKS KQKKKKSKQE AGSNLNFDPS KLSRMQYLLV 201 QLQNTFSFVN GNIILKSQKT LKPNKNGTND NINNHYYNNC NNNNNNINNS 251 NNSNNNNSNN INRNSNHSTN VFSTPEHIQS SINLDKLESL PALDTKGEPS 301 FISPAQFSLL SSAPADNLIL QTPPSPFFQQ TMPIQLPRDA QQEQISPVFS 351 TDVIYMWQTM FNTIENLKEQ VNCLKNEVKQ LNHKFYQQNK PLHNMSTSDS 401 ENFMQQH*

Amino Acid Sequence (or in FASTA format)

       1  MYLEYLQPKL NLMDESSTIS KNFPDYSPNL NTPITSNFNE ETGSDCSLVT
      51  PRIISSSNSN SNSNSNSNSN SNSGSIDENE LNNSNSSSSS ARQIRKKWKE
     101  PEDIAFITTI MNNSQLLTFV EYFKPMKNFW KKISKILFQQ YGYERNSRQC
     151  HDRFKVLYTK SLKVHPSKKS KQKKKKSKQE AGSNLNFDPS KLSRMQYLLV
     201  QLQNTFSFVN GNIILKSQKT LKPNKNGTND NINNHYYNNC NNNNNNINNS
     251  NNSNNNNSNN INRNSNHSTN VFSTPEHIQS SINLDKLESL PALDTKGEPS
     301  FISPAQFSLL SSAPADNLIL QTPPSPFFQQ TMPIQLPRDA QQEQISPVFS
     351  TDVIYMWQTM FNTIENLKEQ VNCLKNEVKQ LNHKFYQQNK PLHNMSTSDS
     401  ENFMQQH*

external links for Rpi1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rpi1p

1)	Kim JH and Powers S (1991) Overexpression of RPI1, a novel inhibitor of the yeast Ras-cyclic AMP pathway, down-regulates normal but not mutationally activated ras function. Mol Cell Biol 11(8):3894-904
2)	Sobering AK, et al. (2002) Yeast Rpi1 is a putative transcriptional regulator that contributes to preparation for stationary phase. Eukaryot Cell 1(1):56-65
3)	Puria R, et al. (2009) Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation. FEMS Yeast Res 9(8):1161-71
4)	Chin BL, et al. (2012) Genetic variation in Saccharomyces cerevisiae: circuit diversification in a signal transduction network. Genetics 192(4):1523-32
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41