NUP159/YIL115C Protein Information

Standard Name	Nup159p 1
Systematic Name	Yil115cp
Alias	Nup158p , Rat7p 2
ORF Classification	Verified
Description	FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) (10, 11, 12, 13, 3, 4, 5, 6, 7, 8, 9)
Name Description	NUclear Pore 14

Experimental Data
Molecules/cell	1230 15

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,460
Molecular Weight (Da)	158,906
Isoelectric Point (pI)	4.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nup159p (InterPro)
Physical Interactions	There are 105 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000036C5D \| P40477 MIPS: YIL115C NCBI: 333361500 \| 354459535 \| 354459538 \| 354459541 \| 354459544 \| 380765156 \| 380765158 \| 383875492 \| 383875495 \| 383875498 \| 558691 \| 58177360 \| 6322076 \| 731862 \| 806505 \| NP_012151.1 GenBank/EMBL/DDBJ: DAA08438.1 \| L40634 \| Z38125

Amino Acid Sequence (or in FASTA format)
1 MSSLKDEVPT ETSEDFGFKF LGQKQILPSF NEKLPFASLQ NLDISNSKSL 51 FVAASGSKAV VGELQLLRDH ITSDSTPLTF KWEKEIPDVI FVCFHGDQVL 101 VSTRNALYSL DLEELSEFRT VTSFEKPVFQ LKNVNNTLVI LNSVNDLSAL 151 DLRTKSTKQL AQNVTSFDVT NSQLAVLLKD RSFQSFAWRN GEMEKQFEFS 201 LPSELEELPV EEYSPLSVTI LSPQDFLAVF GNVISETDDE VSYDQKMYII 251 KHIDGSASFQ ETFDITPPFG QIVRFPYMYK VTLSGLIEPD ANVNVLASSC 301 SSEVSIWDSK QVIEPSQDSE RAVLPISEET DKDTNPIGVA VDVVTSGTIL 351 EPCSGVDTIE RLPLVYILNN EGSLQIVGLF HVAAIKSGHY SINLESLEHE 401 KSLSPTSEKI PIAGQEQEEK KKNNESSKAL SENPFTSANT SGFTFLKTQP 451 AAANSLQSQS SSTFGAPSFG SSAFKIDLPS VSSTSTGVAS SEQDATDPAS 501 AKPVFGKPAF GAIAKEPSTS EYAFGKPSFG APSFGSGKSS VESPASGSAF 551 GKPSFGTPSF GSGNSSVEPP ASGSAFGKPS FGTPSFGSGN SSAEPPASGS 601 AFGKPSFGTS AFGTASSNET NSGSIFGKAA FGSSSFAPAN NELFGSNFTI 651 SKPTVDSPKE VDSTSPFPSS GDQSEDESKS DVDSSSTPFG TKPNTSTKPK 701 TNAFDFGSSS FGSGFSKALE SVGSDTTFKF GTQASPFSSQ LGNKSPFSSF 751 TKDDTENGSL SKGSTSEIND DNEEHESNGP NVSGNDLTDS TVEQTSSTRL 801 PETPSDEDGE VVEEEAQKSP IGKLTETIKK SANIDMAGLK NPVFGNHVKA 851 KSESPFSAFA TNITKPSSTT PAFSFGNSTM NKSNTSTVSP MEEADTKETS 901 EKGPITLKSV ENPFLPAKEE RTGESSKKDH NDDPKDGYVS GSEISVRTSE 951 SAFDTTANEE IPKSQDVNNH EKSETDPKYS QHAVVDHDNK SKEMNETSKN 1001 NERSGQPNHG VQGDGIALKK DNEKENFDSN MAIKQFEDHQ SSEEDASEKD 1051 SRQSSEVKES DDNMSLNSDR DESISESYDK LEDINTDELP HGGEAFKARE 1101 VSASADFDVQ TSLEDNYAES GIQTDLSESS KENEVQTDAI PVKHNSTQTV 1151 KKEAVDNGLQ TEPVETCNFS VQTFEGDENY LAEQCKPKQL KEYYTSAKVS 1201 NIPFVSQNST LRLIESTFQT VEAEFTVLME NIRNMDTFFT DQSSIPLVKR 1251 TVRSINNLYT WRIPEAEILL NIQNNIKCEQ MQITNANIQD LKEKVTDYVR 1301 KDIAQITEDV ANAKEEYLFL MHFDDASSGY VKDLSTHQFR MQKTLRQKLF 1351 DVSAKINHTE ELLNILKLFT VKNKRLDDNP LVAKLAKESL ARDGLLKEIK 1401 LLREQVSRLQ LEEKGKKASS FDASSSITKD MKGFKVVEVG LAMNTKKQIG 1451 DFFKNLNMAK *

Amino Acid Sequence (or in FASTA format)

       1  MSSLKDEVPT ETSEDFGFKF LGQKQILPSF NEKLPFASLQ NLDISNSKSL
      51  FVAASGSKAV VGELQLLRDH ITSDSTPLTF KWEKEIPDVI FVCFHGDQVL
     101  VSTRNALYSL DLEELSEFRT VTSFEKPVFQ LKNVNNTLVI LNSVNDLSAL
     151  DLRTKSTKQL AQNVTSFDVT NSQLAVLLKD RSFQSFAWRN GEMEKQFEFS
     201  LPSELEELPV EEYSPLSVTI LSPQDFLAVF GNVISETDDE VSYDQKMYII
     251  KHIDGSASFQ ETFDITPPFG QIVRFPYMYK VTLSGLIEPD ANVNVLASSC
     301  SSEVSIWDSK QVIEPSQDSE RAVLPISEET DKDTNPIGVA VDVVTSGTIL
     351  EPCSGVDTIE RLPLVYILNN EGSLQIVGLF HVAAIKSGHY SINLESLEHE
     401  KSLSPTSEKI PIAGQEQEEK KKNNESSKAL SENPFTSANT SGFTFLKTQP
     451  AAANSLQSQS SSTFGAPSFG SSAFKIDLPS VSSTSTGVAS SEQDATDPAS
     501  AKPVFGKPAF GAIAKEPSTS EYAFGKPSFG APSFGSGKSS VESPASGSAF
     551  GKPSFGTPSF GSGNSSVEPP ASGSAFGKPS FGTPSFGSGN SSAEPPASGS
     601  AFGKPSFGTS AFGTASSNET NSGSIFGKAA FGSSSFAPAN NELFGSNFTI
     651  SKPTVDSPKE VDSTSPFPSS GDQSEDESKS DVDSSSTPFG TKPNTSTKPK
     701  TNAFDFGSSS FGSGFSKALE SVGSDTTFKF GTQASPFSSQ LGNKSPFSSF
     751  TKDDTENGSL SKGSTSEIND DNEEHESNGP NVSGNDLTDS TVEQTSSTRL
     801  PETPSDEDGE VVEEEAQKSP IGKLTETIKK SANIDMAGLK NPVFGNHVKA
     851  KSESPFSAFA TNITKPSSTT PAFSFGNSTM NKSNTSTVSP MEEADTKETS
     901  EKGPITLKSV ENPFLPAKEE RTGESSKKDH NDDPKDGYVS GSEISVRTSE
     951  SAFDTTANEE IPKSQDVNNH EKSETDPKYS QHAVVDHDNK SKEMNETSKN
    1001  NERSGQPNHG VQGDGIALKK DNEKENFDSN MAIKQFEDHQ SSEEDASEKD
    1051  SRQSSEVKES DDNMSLNSDR DESISESYDK LEDINTDELP HGGEAFKARE
    1101  VSASADFDVQ TSLEDNYAES GIQTDLSESS KENEVQTDAI PVKHNSTQTV
    1151  KKEAVDNGLQ TEPVETCNFS VQTFEGDENY LAEQCKPKQL KEYYTSAKVS
    1201  NIPFVSQNST LRLIESTFQT VEAEFTVLME NIRNMDTFFT DQSSIPLVKR
    1251  TVRSINNLYT WRIPEAEILL NIQNNIKCEQ MQITNANIQD LKEKVTDYVR
    1301  KDIAQITEDV ANAKEEYLFL MHFDDASSGY VKDLSTHQFR MQKTLRQKLF
    1351  DVSAKINHTE ELLNILKLFT VKNKRLDDNP LVAKLAKESL ARDGLLKEIK
    1401  LLREQVSRLQ LEEKGKKASS FDASSSITKD MKGFKVVEVG LAMNTKKQIG
    1451  DFFKNLNMAK *

external links for Nup159p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Nup159p

1)	Kraemer DM, et al. (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J Biol Chem 270(32):19017-21
2)	Gorsch LC, et al. (1995) A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129(4):939-55
3)	Strasser K, et al. (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol 150(4):695-706
4)	Hodge CA, et al. (1999) Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells. EMBO J 18(20):5778-88
5)	Rout MP, et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
6)	Stage-Zimmermann T, et al. (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
7)	Bailer SM, et al. (2000) Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J Biol Chem 275(31):23540-8
8)	Grosshans H, et al. (2001) Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export. J Cell Biol 153(4):745-62
9)	Strawn LA, et al. (2004) Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol 6(3):197-206
10)	Weirich CS, et al. (2004) The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore. Mol Cell 16(5):749-60
11)	Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
12)	Patel SS, et al. (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
13)	Noble KN, et al. (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77
14)	Davis LI and Fink GR (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
15)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41