ICE2/YIL090W Protein Information

Standard Name	Ice2p 1
Systematic Name	Yil090wp
ORF Classification	Verified
Description	Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells (1, 2, 3)
Name Description	Inheritance of Cortical ER 1

Experimental Data
Molecules/cell	606 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	491
Molecular Weight (Da)	56,332
Isoelectric Point (pI)	9.14

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ice2p (InterPro)
Transmembrane Domains	There are 8 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B41F \| P40499 MIPS: YIL090W NCBI: 51012737 \| 577124 \| 6322101 \| 731841 \| NP_012176.1 GenBank/EMBL/DDBJ: DAA08463.1 \| AY692643 \| Z46728

Amino Acid Sequence (or in FASTA format)
1 MTSLSKSFMQ SGRICAACFY LLFTLLSIPI SFKVGGLECG LSFTVTLFTL 51 YFITTTLNVL ARRHGGRLYI FFTNCLYYSQ HFIIASLLYL FLSGFSNDEL 101 GNVLKNKYNE SESFLEALKN SLNSNQINYV LYYYYYRFVV QPWQFVLTKS 151 TPFFTLSEGF FTILAIQAVG ETNRWLSNDL NSNTWIISSL LTSGGVITAS 201 LYYLYRIYVT PIWPLSIQTA SLLGLVLSMV CGLGLYGIVS QKGSVIESSL 251 FFAYIVRCIY EISPKLATTA TDEILNLFKD VWQKHQRNLP TADNLLCYFH 301 NVILKNAEVL WGSFIPRGRK KTGDFHDKLI SILSFEKVSL ISKPFWKFFK 351 NFTFSVPLSI NEFCQVTIKM ASESVSPAIV INLCFRVLMF YSATRIIPAL 401 QRKNDKQLRK SRRIMKGLYW YSPCILIAMY THLILQYSGE LKKDLCIWGC 451 SEKWFGVDQP EIIVDSWGFW NWCNIFCTIL VYATELIGSG S*

Amino Acid Sequence (or in FASTA format)

       1  MTSLSKSFMQ SGRICAACFY LLFTLLSIPI SFKVGGLECG LSFTVTLFTL
      51  YFITTTLNVL ARRHGGRLYI FFTNCLYYSQ HFIIASLLYL FLSGFSNDEL
     101  GNVLKNKYNE SESFLEALKN SLNSNQINYV LYYYYYRFVV QPWQFVLTKS
     151  TPFFTLSEGF FTILAIQAVG ETNRWLSNDL NSNTWIISSL LTSGGVITAS
     201  LYYLYRIYVT PIWPLSIQTA SLLGLVLSMV CGLGLYGIVS QKGSVIESSL
     251  FFAYIVRCIY EISPKLATTA TDEILNLFKD VWQKHQRNLP TADNLLCYFH
     301  NVILKNAEVL WGSFIPRGRK KTGDFHDKLI SILSFEKVSL ISKPFWKFFK
     351  NFTFSVPLSI NEFCQVTIKM ASESVSPAIV INLCFRVLMF YSATRIIPAL
     401  QRKNDKQLRK SRRIMKGLYW YSPCILIAMY THLILQYSGE LKKDLCIWGC
     451  SEKWFGVDQP EIIVDSWGFW NWCNIFCTIL VYATELIGSG S*

external links for Ice2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Ice2p

1)	Estrada de Martin P, et al. (2005) Ice2p is important for the distribution and structure of the cortical ER network in Saccharomyces cerevisiae. J Cell Sci 118(Pt 1):65-77
2)	Murthi A and Hopper AK (2005) Genome-wide screen for inner nuclear membrane protein targeting in Saccharomyces cerevisiae: roles for N-acetylation and an integral membrane protein. Genetics 170(4):1553-60
3)	North M, et al. (2012) Genome-Wide Functional Profiling Identifies Genes and Processes Important for Zinc-Limited Growth of Saccharomyces cerevisiae. PLoS Genet 8(6):e1002699
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41