RHR2/YIL053W Protein Information

Standard Name	Rhr2p
Systematic Name	Yil053wp
Alias	Gpp1p 1
ORF Classification	Verified
Description	Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Related to HoR2 6

Experimental Data
Molecules/cell	193000 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	250
Molecular Weight (Da)	27,947
Isoelectric Point (pI)	5.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rhr2p (InterPro)
Physical Interactions	There are 49 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168DE5 \| P41277 MIPS: YIL053W NCBI: 1236750 \| 157835990 \| 2507038 \| 557815 \| 86558907 \| NP_012211.2 \| NM_001179403.1 GenBank/EMBL/DDBJ: DAA08494.1 \| D50471 \| Z38060
External Classifications	EC: 3.1.3.- [Phosphoric Monoester Hydrolases] EC: 3.1.3.21 [Glycerol-1-phosphatase]

Amino Acid Sequence (or in FASTA format)
1 MPLTTKPLSL KINAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI 51 HISHGWRTYD AIAKFAPDFA DEEYVNKLEG EIPEKYGEHS IEVPGAVKLC 101 NALNALPKEK WAVATSGTRD MAKKWFDILK IKRPEYFITA NDVKQGKPHP 151 EPYLKGRNGL GFPINEQDPS KSKVVVFEDA PAGIAAGKAA GCKIVGIATT 201 FDLDFLKEKG CDIIVKNHES IRVGEYNAET DEVELIFDDY LYAKDDLLKW 251 *

Amino Acid Sequence (or in FASTA format)

       1  MPLTTKPLSL KINAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI
      51  HISHGWRTYD AIAKFAPDFA DEEYVNKLEG EIPEKYGEHS IEVPGAVKLC
     101  NALNALPKEK WAVATSGTRD MAKKWFDILK IKRPEYFITA NDVKQGKPHP
     151  EPYLKGRNGL GFPINEQDPS KSKVVVFEDA PAGIAAGKAA GCKIVGIATT
     201  FDLDFLKEKG CDIIVKNHES IRVGEYNAET DEVELIFDDY LYAKDDLLKW
     251  *

external links for Rhr2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rhr2p

1)	Norbeck J, et al. (1996) Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway. J Biol Chem 271(23):13875-81
2)	Norbeck J and Blomberg A (1997) Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway. J Biol Chem 272(9):5544-54
3)	Pahlman AK, et al. (2001) The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress. J Biol Chem 276(5):3555-63
4)	Nevoigt E and Stahl U (1997) Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 21(3):231-41
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Hirayama T, et al. (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41