MET30/YIL046W Protein Information Help

Standard Name Met30p 1
Systematic Name Yil046wp
Alias Zrg11p 2
ORF Classification Verified
Description F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus (3, 4)
Name Description METhionine requiring
Experimental Data
Molecules/cell 217 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 640
Molecular Weight (Da) 72,835
Isoelectric Point (pI) 7.56

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Met30p (InterPro)
Physical Interactions There are 64 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012F726 | P39014
MIPS: YIL046W
NCBI: 432494 | 600002 | 6322143 | 730077 | NP_012218.1
GenBank/EMBL/DDBJ: DAA08502.1 | L26505 | Z46861
Amino Acid Sequence (or in FASTA format)
 
       1  MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD
      51  LAQGSSGKKK MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP
     101  DIQFSPTHTA CYKQDLKRTQ EINANIAKLP LQEQSDIHHI ISKYSNSNDK
     151  IRKLILDGIL STSCFPQLSY ISSLVTHMIK IDFISILPQE LSLKILSYLD
     201  CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG WGLPLLHMKR
     251  ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG
     301  HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF
     351  DDRKLITGSL DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA
     401  DKTVKVWHVE SRTCYTLRGH TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI
     451  RTNSCLKVFR GHVGQVQKII PLTIKDVENL ATDNTSDGSS PQDDPTMTDG
     501  ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK TGKCIRTQFG
     551  HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT
     601  QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND *

external links for Met30p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Met30p
1) Thomas D, et al.  (1995) Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol 15(12):6526-34
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) Rouillon A, et al.  (2000) Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex. EMBO J 19(2):282-94
4) Smothers DB, et al.  (2000) The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability. Mol Cell Biol 20(21):7845-52
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41