CBR1/YIL043C Protein Information

Standard Name	Cbr1p
Systematic Name	Yil043cp
Alias	Cbr5p
ORF Classification	Verified
Description	Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia (1, 2, 3, 4)
Name Description	Cytochrome b Reductase 2

Experimental Data
Molecules/cell	4820 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	284
Molecular Weight (Da)	31,494
Isoelectric Point (pI)	9.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cbr1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 37 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000693A78 \| P38626 MIPS: YIL043C NCBI: 187608852 \| 461338 \| 585542 \| 600005 \| 86558908 \| NP_012221.2 GenBank/EMBL/DDBJ: DAA08505.1 \| Z28365 \| Z46861
External Classifications	EC: 1.6.2.2 [Cytochrome-b5 reductase]

Amino Acid Sequence (or in FASTA format)
1 MAIDAQKLVV VIVIVVVPLL FKFIIGPKTK PVLDPKRNDF QSFPLVEKTI 51 LTHNTSMYKF GLPHADDVLG LPIGQHIVIK ANINGKDITR SYTPTSLDGD 101 TKGNFELLVK SYPTGNVSKM IGELKIGDSI QIKGPRGNYH YERNCRSHLG 151 MIAGGTGIAP MYQIMKAIAM DPHDTTKVSL VFGNVHEEDI LLKKELEALV 201 AMKPSQFKIV YYLDSPDRED WTGGVGYITK DVIKEHLPAA TMDNVQILIC 251 GPPAMVASVR RSTVDLGFRR SKPLSKMEDQ VFVF*

Amino Acid Sequence (or in FASTA format)

       1  MAIDAQKLVV VIVIVVVPLL FKFIIGPKTK PVLDPKRNDF QSFPLVEKTI
      51  LTHNTSMYKF GLPHADDVLG LPIGQHIVIK ANINGKDITR SYTPTSLDGD
     101  TKGNFELLVK SYPTGNVSKM IGELKIGDSI QIKGPRGNYH YERNCRSHLG
     151  MIAGGTGIAP MYQIMKAIAM DPHDTTKVSL VFGNVHEEDI LLKKELEALV
     201  AMKPSQFKIV YYLDSPDRED WTGGVGYITK DVIKEHLPAA TMDNVQILIC
     251  GPPAMVASVR RSTVDLGFRR SKPLSKMEDQ VFVF*

external links for Cbr1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Cbr1p

1)	Kubota S, et al. (1977) Studies on the microsomal electron-transport system of anaerobically grown yeast. IV. Purification and characterization of NADH-cytochrome b5 reductase. J Biochem 81(1):187-95
2)	Csukai M, et al. (1994) Isolation and complete sequence of CBR, a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiae. Eur J Biochem 219(1-2):441-8
3)	Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
4)	Nussenzveig RH, et al. (2006) A novel mutation of the cytochrome-b5 reductase gene in an Indian patient: the molecular basis of type I methemoglobinemia. Haematologica 91(11):1542-5
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41