CKA1/YIL035C Protein Information

Standard Name	Cka1p
Systematic Name	Yil035cp
ORF Classification	Verified
Description	Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching (1, 2, 3, 4, 5)
Name Description	Casein Kinase Alpha subunit
Gene Product	CK2 subunit 1

Experimental Data
Molecules/cell	7180 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	372
Molecular Weight (Da)	44,667
Isoelectric Point (pI)	9.22

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cka1p (InterPro)
Physical Interactions	There are 277 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DE5 \| P15790 MIPS: YIL035C NCBI: 125259 \| 171324 \| 3448 \| 45270488 \| 600013 \| 6322154 \| NP_012229.1 GenBank/EMBL/DDBJ: DAA08513.1 \| AY558299 \| M22473 \| X62630 \| Z46861
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK 51 YSEVFQGVKL DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP 101 FQKDQYYTNQ KEDVLKFIRP YIFDQPHNGH ANIIHLFDII KDPISKTPAL 151 VFEYVDNVDF RILYPKLTDL EIRFYMFELL KALDYCHSMG IMHRDVKPHN 201 VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE LLVDYRMYDY 251 SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY 301 EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE 351 RLTAKEAMGH PWFAPIREQI EK*

Amino Acid Sequence (or in FASTA format)

       1  MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK
      51  YSEVFQGVKL DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP
     101  FQKDQYYTNQ KEDVLKFIRP YIFDQPHNGH ANIIHLFDII KDPISKTPAL
     151  VFEYVDNVDF RILYPKLTDL EIRFYMFELL KALDYCHSMG IMHRDVKPHN
     201  VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE LLVDYRMYDY
     251  SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY
     301  EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE
     351  RLTAKEAMGH PWFAPIREQI EK*

external links for Cka1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cka1p

1)	Chen-Wu JL, et al. (1988) Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II. Mol Cell Biol 8(11):4981-90
2)	Marin O, et al. (1999) Tyrosine versus serine/threonine phosphorylation by protein kinase casein kinase-2. A study with peptide substrates derived from immunophilin Fpr3. J Biol Chem 274(41):29260-5
3)	Ackermann K, et al. (2001) Genes targeted by protein kinase CK2: a genome-wide expression array analysis in yeast. Mol Cell Biochem 227(1-2):59-66
4)	Ahmed K, et al. (2002) Joining the cell survival squad: an emerging role for protein kinase CK2. Trends Cell Biol 12(5):226-30
5)	Li J, et al. (2012) Regulation of Budding Yeast Mating-Type Switching Donor Preference by the FHA Domain of Fkh1. PLoS Genet 8(4):e1002630
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41