HIS6/YIL020C Protein Information

Standard Name	His6p 1
Systematic Name	Yil020cp
ORF Classification	Verified
Description	Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts (1, 2, 3)
Name Description	HIStidine requiring 1

Experimental Data
Molecules/cell	6490 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	261
Molecular Weight (Da)	29,556
Isoelectric Point (pI)	5.73

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with His6p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012C7EB \| P40545 MIPS: YIL020C NCBI: 110590811 \| 2584817 \| 398364475 \| 45269609 \| 599979 \| 731787 \| NP_012244.3 GenBank/EMBL/DDBJ: DAA08525.1 \| AY557859 \| X87341 \| Z46881
External Classifications	EC: 5.3.1.16 [1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase]

Amino Acid Sequence (or in FASTA format)
1 MTKFIGCIDL HNGEVKQIVG GTLTSKKEDV PKTNFVSQHP SSYYAKLYKD 51 RDVQGCHVIK LGPNNDDAAR EALQESPQFL QVGGGINDTN CLEWLKWASK 101 VIVTSWLFTK EGHFQLKRLE RLTELCGKDR IVVDLSCRKT QDGRWIVAMN 151 KWQTLTDLEL NADTFRELRK YTNEFLIHAA DVEGLCGGID ELLVSKLFEW 201 TKDYDDLKIV YAGGAKSVDD LKLVDELSHG KVDLTFGSSL DIFGGNLVKF 251 EDCCRWNEKQ G*

Amino Acid Sequence (or in FASTA format)

       1  MTKFIGCIDL HNGEVKQIVG GTLTSKKEDV PKTNFVSQHP SSYYAKLYKD
      51  RDVQGCHVIK LGPNNDDAAR EALQESPQFL QVGGGINDTN CLEWLKWASK
     101  VIVTSWLFTK EGHFQLKRLE RLTELCGKDR IVVDLSCRKT QDGRWIVAMN
     151  KWQTLTDLEL NADTFRELRK YTNEFLIHAA DVEGLCGGID ELLVSKLFEW
     201  TKDYDDLKIV YAGGAKSVDD LKLVDELSHG KVDLTFGSSL DIFGGNLVKF
     251  EDCCRWNEKQ G*

external links for His6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for His6p

1)	Fani R, et al. (1997) Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes. Gene 197(1-2):9-17
2)	Pearce DA and Sherman F (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9
3)	Alifano P, et al. (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41