IMD2/YHR216W Protein Information Help

Standard Name Imd2p 1
Systematic Name Yhr216wp
Alias Pur5p 2
ORF Classification Verified
Description Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication (1, 3, 4, 5, 6)
Name Description IMP Dehydrogenase 1
Experimental Data
Molecules/cell 7870 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 523
Molecular Weight (Da) 56,530
Isoelectric Point (pI) 8.59

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Imd2p (InterPro)
Physical Interactions There are 49 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000534E8 | P38697
MIPS: YHR216W
NCBI: 398365225 | 458916 | 729848 | NP_012088.3 | NM_001179347.3
GenBank/EMBL/DDBJ: AAB69728.1 | DAA06915.1 | U00029
External Classifications EC: 1.1.1.205 [IMP dehydrogenase]
Amino Acid Sequence (or in FASTA format)
       1  MAAIRDYKTA LDFTKSLPRP DGLSVQELMD SKIRGGLTYN DFLILPGLVD
      51  FASSEVSLQT KLTRNITLNI PLVSSPMDTV TESEMATFMA LLGGIGFIHH
     101  NCTPEDQADM VRRVKNYENG FINNPIVISP TTTVGEAKSM KEKYGFAGFP
     151  VTTDGKRNAK LVGVITSRDI QFVEDNSLLV QDVMTKNPVT GAQGITLSEG
     201  NEILKKIKKG RLLVVDEKGN LVSMLSRTDL MKNQNYPLAS KSANTKQLLC
     251  GASIGTMDAD KERLRLLVKA GLDVVILDSS QGNSIFELNM LKWVKESFPG
     301  LEVIAGNVVT REQAANLIAA GADGLRIGMG TGSICITQEV MACGRPQGTA
     351  VYNVCEFANQ FGVPCMADGG VQNIGHITKA LALGSSTVMM GGMLAGTTES
     401  PGEYFYQDGK RLKAYRGMGS IDAMQKTGTK GNASTSRYFS ESDSVLVAQG
     451  VSGAVVDKGS IKKFIPYLYN GLQHSCQDIG CRSLTLLKNN VQRGKVRFEF
     501  RTASAQLEGG VHNLHSYEKR LHN*                            

external links for Imd2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
YGOB CYC2008 (complexes) MIPS YPL+
YOGY Complexome Pfam domains YeastGFP

DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Imd2p
1) Escobar-Henriques M and Daignan-Fornier B  (2001) Transcriptional regulation of the yeast gmp synthesis pathway by its end products. J Biol Chem 276(2):1523-30
2) Shaw RJ and Reines D  (2000) Saccharomyces cerevisiae transcription elongation mutants are defective in PUR5 induction in response to nucleotide depletion. Mol Cell Biol 20(20):7427-37
3) Shaw RJ, et al.  (2001) Regulation of an IMP dehydrogenase gene and its overexpression in drug-sensitive transcription elongation mutants of yeast. J Biol Chem 276(35):32905-16
4) Hyle JW, et al.  (2003) Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast. J Biol Chem 278(31):28470-8
5) Escobar-Henriques M, et al.  (2003) Transcription initiation of the yeast IMD2 gene is abolished in response to nutrient limitation through a sequence in its coding region. Mol Cell Biol 23(17):6279-90
6) Katju V, et al.  (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41