SCH9/YHR205W Protein Information

Standard Name	Sch9p 1
Systematic Name	Yhr205wp
Alias	Kom1p , Hrm2p 2
ORF Classification	Verified
Description	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan (2, 3, 4, 5, 6, 7, 8, 9)

Experimental Data
Molecules/cell	3850 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	824
Molecular Weight (Da)	91,811
Isoelectric Point (pI)	6.04

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sch9p (InterPro)
Physical Interactions	There are 48 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000016834E \| P11792 MIPS: YHR205W NCBI: 114152871 \| 4426 \| 458923 \| 5279 \| 6321999 \| 730723 \| NP_012075.1 GenBank/EMBL/DDBJ: DAA06898.1 \| U00029 \| X12560 \| X57629
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MMNFFTSKSS NQDTGFSSQH QHPNGQNNGN NNSSTAGNDN GYPCKLVSSG 51 PCASSNNGAL FTNFTLQTAT PTTAISQDLY AMGTTGITSE NALFQMKSMN 101 NGISSVNNNN SNTPTIITTS QEETNAGNVH GDTGGNSLQN SEDDNFSSSS 151 TTKCLLSSTS SLSINQREAA AAAYGPDTDI PRGKLEVTII EARDLVTRSK 201 DSQPYVVCTF ESSEFISNGP ESLGAINNNN NNNNNNQHNQ NQHINNNNEN 251 TNPDAASQHH NNNSGWNGSQ LPSIKEHLKK KPLYTHRSSS QLDQLNSCSS 301 VTDPSKRSSN SSSGSSNGPK NDSSHPIWHH KTTFDVLGSH SELDISVYDA 351 AHDHMFLGQV RLYPMIHNLA HASQHQWHSL KPRVIDEVVS GDILIKWTYK 401 QTKKRHYGPQ DFEVLRLLGK GTFGQVYQVK KKDTQRIYAM KVLSKKVIVK 451 KNEIAHTIGE RNILVTTASK SSPFIVGLKF SFQTPTDLYL VTDYMSGGEL 501 FWHLQKEGRF SEDRAKFYIA ELVLALEHLH DNDIVYRDLK PENILLDANG 551 NIALCDFGLS KADLKDRTNT FCGTTEYLAP ELLLDETGYT KMVDFWSLGV 601 LIFEMCCGWS PFFAENNQKM YQKIAFGKVK FPRDVLSQEG RSFVKGLLNR 651 NPKHRLGAID DGRELRAHPF FADIDWEALK QKKIPPPFKP HLVSETDTSN 701 FDPEFTTAST SYMNKHQPMM TATPLSPAMQ AKFAGFTFVD ESAIDEHVNN 751 NRKFLQNSYF MEPGSFIPGN PNLPPDEDVI DDDGDEDIND GFNQEKNMNN 801 SHSQMDFDGD QHMDDEFVSG RFEI*

Amino Acid Sequence (or in FASTA format)

       1  MMNFFTSKSS NQDTGFSSQH QHPNGQNNGN NNSSTAGNDN GYPCKLVSSG
      51  PCASSNNGAL FTNFTLQTAT PTTAISQDLY AMGTTGITSE NALFQMKSMN
     101  NGISSVNNNN SNTPTIITTS QEETNAGNVH GDTGGNSLQN SEDDNFSSSS
     151  TTKCLLSSTS SLSINQREAA AAAYGPDTDI PRGKLEVTII EARDLVTRSK
     201  DSQPYVVCTF ESSEFISNGP ESLGAINNNN NNNNNNQHNQ NQHINNNNEN
     251  TNPDAASQHH NNNSGWNGSQ LPSIKEHLKK KPLYTHRSSS QLDQLNSCSS
     301  VTDPSKRSSN SSSGSSNGPK NDSSHPIWHH KTTFDVLGSH SELDISVYDA
     351  AHDHMFLGQV RLYPMIHNLA HASQHQWHSL KPRVIDEVVS GDILIKWTYK
     401  QTKKRHYGPQ DFEVLRLLGK GTFGQVYQVK KKDTQRIYAM KVLSKKVIVK
     451  KNEIAHTIGE RNILVTTASK SSPFIVGLKF SFQTPTDLYL VTDYMSGGEL
     501  FWHLQKEGRF SEDRAKFYIA ELVLALEHLH DNDIVYRDLK PENILLDANG
     551  NIALCDFGLS KADLKDRTNT FCGTTEYLAP ELLLDETGYT KMVDFWSLGV
     601  LIFEMCCGWS PFFAENNQKM YQKIAFGKVK FPRDVLSQEG RSFVKGLLNR
     651  NPKHRLGAID DGRELRAHPF FADIDWEALK QKKIPPPFKP HLVSETDTSN
     701  FDPEFTTAST SYMNKHQPMM TATPLSPAMQ AKFAGFTFVD ESAIDEHVNN
     751  NRKFLQNSYF MEPGSFIPGN PNLPPDEDVI DDDGDEDIND GFNQEKNMNN
     801  SHSQMDFDGD QHMDDEFVSG RFEI*

external links for Sch9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sch9p

1)	Toda T, et al. (1988) SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits. Genes Dev 2(5):517-27
2)	Prusty R and Keil RL (2004) SCH9, a putative protein kinase from Saccharomyces cerevisiae, affects HOT1-stimulated recombination. Mol Genet Genomics 272(3):264-74
3)	Crauwels M, et al. (1997) The Sch9 protein kinase in the yeast Saccharomyces cerevisiae controls cAPK activity and is required for nitrogen activation of the fermentable-growth-medium-induced (FGM) pathway. Microbiology 143 ( Pt 8)():2627-37
4)	di Blasi F, et al. (1993) The SCH9 protein kinase mRNA contains a long 5' leader with a small open reading frame. Yeast 9(1):21-32
5)	Morano KA and Thiele DJ (1999) The Sch9 protein kinase regulates Hsp90 chaperone complex signal transduction activity in vivo. EMBO J 18(21):5953-62
6)	Fabrizio P, et al. (2001) Regulation of longevity and stress resistance by Sch9 in yeast. Science 292(5515):288-90
7)	Urban J, et al. (2007) Sch9 is a major target of TORC1 in Saccharomyces cerevisiae. Mol Cell 26(5):663-74
8)	Pascual-Ahuir A and Proft M (2007) The Sch9 kinase is a chromatin-associated transcriptional activator of osmostress-responsive genes. EMBO J 26(13):3098-108
9)	Huang X, et al. (2012) Down-regulating sphingolipid synthesis increases yeast lifespan. PLoS Genet 8(2):e1002493
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41