NVJ1/YHR195W Protein Information

Standard Name	Nvj1p
Systematic Name	Yhr195wp
Alias	Vab36p
ORF Classification	Verified
Description	Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) (1, 2, 3)
Name Description	Nucleus-Vacuole Junction 1

Experimental Data
Molecules/cell	1900 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	321
Molecular Weight (Da)	36,421
Isoelectric Point (pI)	4.9

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nvj1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 18 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2FF \| P38881 MIPS: YHR195W NCBI: 398364995 \| 458931 \| 731764 \| NP_012065.3 GenBank/EMBL/DDBJ: DAA06887.1 \| U00030

Amino Acid Sequence (or in FASTA format)
1 MTRPPLVRGI FSLGLSVAVL KGVEKTVRKH LERQGWIEPQ KVDYELIFTI 51 DRLKNLVDNK REALTAEQPD AGELSWRKVF NFISRQSSEL DTRIYVLILL 101 LSFLLPIAWT VLDGDRETTL EDKDNDCNVD LIENERRLKH YNDGERAVLQ 151 FGKNRSEPII LSYKDMNVLE GEHEFTSKEE HSNSHLTSKS ENALNQVGSE 201 DLLGCHLEKQ LEEDKNEPNG EADGEDDNNR EKDCSSSSEV ESQSKCRKES 251 TAEPDSLSRD TRTTSSLKSS TSFPISFKGS IDLKSLNQPS SLLHIQVSPT 301 KSSNLDAQVN TEQAYSQPFR Y*

Amino Acid Sequence (or in FASTA format)

       1  MTRPPLVRGI FSLGLSVAVL KGVEKTVRKH LERQGWIEPQ KVDYELIFTI
      51  DRLKNLVDNK REALTAEQPD AGELSWRKVF NFISRQSSEL DTRIYVLILL
     101  LSFLLPIAWT VLDGDRETTL EDKDNDCNVD LIENERRLKH YNDGERAVLQ
     151  FGKNRSEPII LSYKDMNVLE GEHEFTSKEE HSNSHLTSKS ENALNQVGSE
     201  DLLGCHLEKQ LEEDKNEPNG EADGEDDNNR EKDCSSSSEV ESQSKCRKES
     251  TAEPDSLSRD TRTTSSLKSS TSFPISFKGS IDLKSLNQPS SLLHIQVSPT
     301  KSSNLDAQVN TEQAYSQPFR Y*

external links for Nvj1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Nvj1p

1)	Pan X, et al. (2000) Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell 11(7):2445-57
2)	Roberts P, et al. (2003) Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae. Mol Biol Cell 14(1):129-41
3)	Millen JI, et al. (2008) The luminal N-terminus of yeast Nvj1 is an inner nuclear membrane anchor. Traffic 9(10):1653-64
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41