GPI16/YHR188C Protein Information

Standard Name	Gpi16p 1
Systematic Name	Yhr188cp
ORF Classification	Verified
Description	Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog (1, 2)
Name Description	GlycosylPhosphatidylInositol anchor biosynthesis 1, 2

Experimental Data
Molecules/cell	1680 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	610
Molecular Weight (Da)	68,771
Isoelectric Point (pI)	5.02

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gpi16p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000016834C \| P38875 MIPS: YHR188C NCBI: 119364587 \| 458939 \| 6321982 \| 731758 \| NP_012058.1 GenBank/EMBL/DDBJ: DAA06880.1 \| U00030

Amino Acid Sequence (or in FASTA format)
1 MILTLAYFML GTLLLGVFAE DTVSQIGIND SLWYPYDEAL VLKPLPNNDL 51 LLSFAFQLQS EPFDPAVSSM SYDAYEHYTT FPRAIPPLLE STATRQFHLR 101 FTRGFWDALS WGQLPHAGKE AGASGVELWS QVQAMDQEQA FHNWKKLSNS 151 LSGLFCSSLN FIDESRTTFP RRSYASDIGA PLFNSTEKLY LMRASLPNEP 201 ICTENLTPFI KLLPTRGKSG LTSLLDGHKL FDSLWNSISL DIATICSEDE 251 DALCHYEMDA RIEMVTHVPS ALARGERPIP KPLDGNTLRC DTDKPFDSYQ 301 CFPLPEPSQT HFKLSQLFAR PINNGNLFAN RPTRICAEVD RSTWTAFLSV 351 DDTIFSTHDN CFDLSNDQNE GGSGYDFILE STDTTKVTPI VPVPIHVSRS 401 LTGNGQDRGG MRIVFHNDND TPVKLIYFES LPWFMRVYLS SLQITSTTSP 451 QLQENDIILD KYYLQAADRK RPGHLEFTML IPANTDIVMT YQFDKALLQF 501 AEYPPDANHG FEIDAAVITV LSLESSSSLY EMRTSTLLLS LSTPDFSMPY 551 NVIILTSTIM GLIFGMLYNL MVKRMVTVEE ADKITLQSGL KYKLLKLKEK 601 FLGKKKTKTD *

Amino Acid Sequence (or in FASTA format)

       1  MILTLAYFML GTLLLGVFAE DTVSQIGIND SLWYPYDEAL VLKPLPNNDL
      51  LLSFAFQLQS EPFDPAVSSM SYDAYEHYTT FPRAIPPLLE STATRQFHLR
     101  FTRGFWDALS WGQLPHAGKE AGASGVELWS QVQAMDQEQA FHNWKKLSNS
     151  LSGLFCSSLN FIDESRTTFP RRSYASDIGA PLFNSTEKLY LMRASLPNEP
     201  ICTENLTPFI KLLPTRGKSG LTSLLDGHKL FDSLWNSISL DIATICSEDE
     251  DALCHYEMDA RIEMVTHVPS ALARGERPIP KPLDGNTLRC DTDKPFDSYQ
     301  CFPLPEPSQT HFKLSQLFAR PINNGNLFAN RPTRICAEVD RSTWTAFLSV
     351  DDTIFSTHDN CFDLSNDQNE GGSGYDFILE STDTTKVTPI VPVPIHVSRS
     401  LTGNGQDRGG MRIVFHNDND TPVKLIYFES LPWFMRVYLS SLQITSTTSP
     451  QLQENDIILD KYYLQAADRK RPGHLEFTML IPANTDIVMT YQFDKALLQF
     501  AEYPPDANHG FEIDAAVITV LSLESSSSLY EMRTSTLLLS LSTPDFSMPY
     551  NVIILTSTIM GLIFGMLYNL MVKRMVTVEE ADKITLQSGL KYKLLKLKEK
     601  FLGKKKTKTD *

external links for Gpi16p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gpi16p

1)	Fraering P, et al. (2001) The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p. Mol Biol Cell 12(10):3295-306
2)	Ohishi K, et al. (2001) PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8. EMBO J 20(15):4088-98
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41