ENO2/YHR174W Protein Information

Standard Name	Eno2p
Systematic Name	Yhr174wp
ORF Classification	Verified
Description	Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication (1, 2, 3)
Name Description	ENO2
Gene Product	enolase 1

Experimental Data
Molecules/cell	2610 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	437
Molecular Weight (Da)	46,914
Isoelectric Point (pI)	5.88

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Eno2p (InterPro)
Physical Interactions	There are 100 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052DE6 \| P00925 MIPS: YHR174W NCBI: 119337 \| 171457 \| 458897 \| 6321968 \| NP_012044.1 GenBank/EMBL/DDBJ: DAA06866.1 \| J01323 \| U00027
External Classifications	EC: 4.2.1.11 [Phosphopyruvate hydratase]

Amino Acid Sequence (or in FASTA format)
1 MAVSKVYARS VYDSRGNPTV EVELTTEKGV FRSIVPSGAS TGVHEALEMR 51 DEDKSKWMGK GVMNAVNNVN NVIAAAFVKA NLDVKDQKAV DDFLLSLDGT 101 ANKSKLGANA ILGVSMAAAR AAAAEKNVPL YQHLADLSKS KTSPYVLPVP 151 FLNVLNGGSH AGGALALQEF MIAPTGAKTF AEAMRIGSEV YHNLKSLTKK 201 RYGASAGNVG DEGGVAPNIQ TAEEALDLIV DAIKAAGHDG KVKIGLDCAS 251 SEFFKDGKYD LDFKNPESDK SKWLTGVELA DMYHSLMKRY PIVSIEDPFA 301 EDDWEAWSHF FKTAGIQIVA DDLTVTNPAR IATAIEKKAA DALLLKVNQI 351 GTLSESIKAA QDSFAANWGV MVSHRSGETE DTFIADLVVG LRTGQIKTGA 401 PARSERLAKL NQLLRIEEEL GDKAVYAGEN FHHGDKL*

Amino Acid Sequence (or in FASTA format)

       1  MAVSKVYARS VYDSRGNPTV EVELTTEKGV FRSIVPSGAS TGVHEALEMR
      51  DEDKSKWMGK GVMNAVNNVN NVIAAAFVKA NLDVKDQKAV DDFLLSLDGT
     101  ANKSKLGANA ILGVSMAAAR AAAAEKNVPL YQHLADLSKS KTSPYVLPVP
     151  FLNVLNGGSH AGGALALQEF MIAPTGAKTF AEAMRIGSEV YHNLKSLTKK
     201  RYGASAGNVG DEGGVAPNIQ TAEEALDLIV DAIKAAGHDG KVKIGLDCAS
     251  SEFFKDGKYD LDFKNPESDK SKWLTGVELA DMYHSLMKRY PIVSIEDPFA
     301  EDDWEAWSHF FKTAGIQIVA DDLTVTNPAR IATAIEKKAA DALLLKVNQI
     351  GTLSESIKAA QDSFAANWGV MVSHRSGETE DTFIADLVVG LRTGQIKTGA
     401  PARSERLAKL NQLLRIEEEL GDKAVYAGEN FHHGDKL*

external links for Eno2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Eno2p

1)	McAlister L and Holland MJ (1982) Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes. J Biol Chem 257(12):7181-8
2)	Entian KD, et al. (1987) Studies on the regulation of enolases and compartmentation of cytosolic enzymes in Saccharomyces cerevisiae. Biochim Biophys Acta 923(2):214-21
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41