NMD3/YHR170W Protein Information

Standard Name	Nmd3p 1
Systematic Name	Yhr170wp
Alias	Src5p
ORF Classification	Verified
Description	Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex (2, 3)
Name Description	Nonsense-Mediated mRNA Decay 1

Experimental Data
Molecules/cell	11900 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	518
Molecular Weight (Da)	59,095
Isoelectric Point (pI)	5.21

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nmd3p (InterPro)
Physical Interactions	There are 110 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005322A \| P38861 MIPS: YHR170W NCBI: 1171738 \| 308153577 \| 458900 \| 6321964 \| 950224 \| NP_012040.1 GenBank/EMBL/DDBJ: DAA06863.1 \| U00027 \| U31376

Amino Acid Sequence (or in FASTA format)
1 MEFTPIDPHQ HQNAATLLCC NCGTPIDGST GLVMCYDCIK LTVDITQGIP 51 REANISFCRN CERFLQPPGQ WIRAELESRE LLAICLRRLK GLTKVRLVDA 101 SFIWTEPHSR RIRIKLTVQG EAMTNTIIQQ TFEVEYIVIA MQCPDCARSY 151 TTNTWRATVQ IRQKVPHKRT FLFLEQLILK HNAHVDTISI SEAKDGLDFF 201 YAQKNHAVKM IDFLNAVVPI KHKKSEELIS QDTHTGASTY KFSYSVEIVP 251 ICKDDLVVLP KKLAKSMGNI SQFVLCSKIS NTVQFMDPTT LQTADLSPSV 301 YWRAPFNALA DVTQLVEFIV LDVDSTGISR GNRVLADITV ARTSDLGVND 351 QVYYVRSHLG GICHAGDSVM GYFIANSNYN SDLFDGLNID YVPDVVLVKK 401 LYQRKSKKSR HWKLKRMAKE HKDIDASLDY NSRAQKQEME RAEKDYELFL 451 QELEEDAELR QSVNLYKNRE ANVPPEEHEM DEDEDEDAPQ INIDELLDEL 501 DEMTLEDGVE NTPVESQQ*

Amino Acid Sequence (or in FASTA format)

       1  MEFTPIDPHQ HQNAATLLCC NCGTPIDGST GLVMCYDCIK LTVDITQGIP
      51  REANISFCRN CERFLQPPGQ WIRAELESRE LLAICLRRLK GLTKVRLVDA
     101  SFIWTEPHSR RIRIKLTVQG EAMTNTIIQQ TFEVEYIVIA MQCPDCARSY
     151  TTNTWRATVQ IRQKVPHKRT FLFLEQLILK HNAHVDTISI SEAKDGLDFF
     201  YAQKNHAVKM IDFLNAVVPI KHKKSEELIS QDTHTGASTY KFSYSVEIVP
     251  ICKDDLVVLP KKLAKSMGNI SQFVLCSKIS NTVQFMDPTT LQTADLSPSV
     301  YWRAPFNALA DVTQLVEFIV LDVDSTGISR GNRVLADITV ARTSDLGVND
     351  QVYYVRSHLG GICHAGDSVM GYFIANSNYN SDLFDGLNID YVPDVVLVKK
     401  LYQRKSKKSR HWKLKRMAKE HKDIDASLDY NSRAQKQEME RAEKDYELFL
     451  QELEEDAELR QSVNLYKNRE ANVPPEEHEM DEDEDEDAPQ INIDELLDEL
     501  DEMTLEDGVE NTPVESQQ*

external links for Nmd3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Nmd3p

1)	He F and Jacobson A (1995) Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen. Genes Dev 9(4):437-54
2)	Ho JH and Johnson AW (1999) NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. Mol Cell Biol 19(3):2389-99
3)	Ho JH, et al. (2000) Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit. J Cell Biol 151(5):1057-66
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41