DBP8/YHR169W Protein Information

Standard Name	Dbp8p 1
Systematic Name	Yhr169wp
ORF Classification	Verified
Description	ATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esf2p (1, 2, 3)
Name Description	Dead Box Protein 1

Experimental Data
Molecules/cell	3500 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	431
Molecular Weight (Da)	47,878
Isoelectric Point (pI)	10.4

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dbp8p (InterPro)
Physical Interactions	There are 61 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053018 \| P38719 MIPS: YHR169W NCBI: 458901 \| 6321963 \| 731740 \| NP_012039.1 GenBank/EMBL/DDBJ: DAA06862.1 \| U00027
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MADFKSLGLS KWLTESLRAM KITQPTAIQK ACIPKILEGR DCIGGAKTGS 51 GKTIAFAGPM LTKWSEDPSG MFGVVLTPTR ELAMQIAEQF TALGSSMNIR 101 VSVIVGGESI VQQALDLQRK PHFIIATPGR LAHHIMSSGD DTVGGLMRAK 151 YLVLDEADIL LTSTFADHLA TCISALPPKD KRQTLLFTAT ITDQVKSLQN 201 APVQKGKPPL FAYQVESVDN VAIPSTLKIE YILVPEHVKE AYLYQLLTCE 251 EYENKTAIIF VNRTMTAEIL RRTLKQLEVR VASLHSQMPQ QERTNSLHRF 301 RANAARILIA TDVASRGLDI PTVELVVNYD IPSDPDVFIH RSGRTARAGR 351 IGDAISFVTQ RDVSRIQAIE DRINKKMTET NKVHDTAVIR KALTKVTKAK 401 RESLMAMQKE NFGERKRQQK KKQNDGKSLR S*

Amino Acid Sequence (or in FASTA format)

       1  MADFKSLGLS KWLTESLRAM KITQPTAIQK ACIPKILEGR DCIGGAKTGS
      51  GKTIAFAGPM LTKWSEDPSG MFGVVLTPTR ELAMQIAEQF TALGSSMNIR
     101  VSVIVGGESI VQQALDLQRK PHFIIATPGR LAHHIMSSGD DTVGGLMRAK
     151  YLVLDEADIL LTSTFADHLA TCISALPPKD KRQTLLFTAT ITDQVKSLQN
     201  APVQKGKPPL FAYQVESVDN VAIPSTLKIE YILVPEHVKE AYLYQLLTCE
     251  EYENKTAIIF VNRTMTAEIL RRTLKQLEVR VASLHSQMPQ QERTNSLHRF
     301  RANAARILIA TDVASRGLDI PTVELVVNYD IPSDPDVFIH RSGRTARAGR
     351  IGDAISFVTQ RDVSRIQAIE DRINKKMTET NKVHDTAVIR KALTKVTKAK
     401  RESLMAMQKE NFGERKRQQK KKQNDGKSLR S*

external links for Dbp8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Dbp8p

1)	Daugeron MC and Linder P (2001) Characterization and mutational analysis of yeast Dbp8p, a putative RNA helicase involved in ribosome biogenesis. Nucleic Acids Res 29(5):1144-55
2)	Grandi P, et al. (2002) 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell 10(1):105-15
3)	Granneman S, et al. (2006) The nucleolar protein Esf2 interacts directly with the DExD/H box RNA helicase, Dbp8, to stimulate ATP hydrolysis. Nucleic Acids Res 34(10):3189-99
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41