DNA2/YHR164C Protein Information 
| Standard Name | Dna2p 1 |
|---|---|
| Systematic Name | Yhr164cp |
| Alias | Web2p |
| ORF Classification | Verified |
| Description | Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress (10, 11, 12, 13, 2, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | DNA synthesis defective 1 |
| Click on image for expanded interactive view |
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| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Dna2p (InterPro) |
| Physical Interactions | There are 31 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI000005304D | P38859 MIPS: YHR164C NCBI: 458906 | 6321958 | 731738 | NP_012034.1 | NM_001179295.1 GenBank/EMBL/DDBJ: DAA06857.1 | U00027 |
| External Classifications | EC: 3.1.-.- [Hydrolases acting on ester bonds ] EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase] |
external links for Dna2p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | YPL+ |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YeastGFP |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastRC Public Image Repository |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | |
| Candida (CandidaDB) | Complexome | YeastRC Structure Prediction (Seattle) | |
| YGOB | DIP | ||
| YOGY | GeneMANIA |
References cited on this page View Complete Literature Guide for Dna2p
| 1) | Dumas LB, et al. (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6 |
| 2) | Budd ME and Campbell JL (2000) The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res 459(3):173-86 |
| 3) | Budd ME and Campbell JL (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17(4):2136-42 |
| 4) | Schlesinger MB and Formosa T (2000) POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics 155(4):1593-606 |
| 5) | Bae SH, et al. (1998) Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem 273(41):26880-90 |
| 6) | Bae SH and Seo YS (2000) Characterization of the enzymatic properties of the yeast dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275(48):38022-31 |
| 7) | Hoopes LL, et al. (2002) Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol 22(12):4136-46 |
| 8) | Choe W, et al. (2002) Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication. Mol Cell Biol 22(12):4202-17 |
| 9) | Kao HI, et al. (2004) Dna2p helicase/nuclease is a tracking protein, like FEN1, for flap cleavage during Okazaki fragment maturation. J Biol Chem 279(49):50840-9 |
| 10) | Kosugi S, et al. (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6 |
| 11) | Manfrini N, et al. (2010) Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem 285(15):11628-37 |
| 12) | Balakrishnan L, et al. (2010) Dna2 exhibits a unique strand end-dependent helicase function. J Biol Chem 285(50):38861-8 |
| 13) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
