WSS1/YHR134W Protein Information

Standard Name	Wss1p
Systematic Name	Yhr134wp
ORF Classification	Verified
Description	Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response (1, 2, 3, 4, 5)
Name Description	Weak Suppressor of Smt3 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	269
Molecular Weight (Da)	30,627
Isoelectric Point (pI)	9.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Wss1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2D6 \| P38838 MIPS: YHR134W NCBI: 500671 \| 6321926 \| 731718 \| NP_012002.1 GenBank/EMBL/DDBJ: DAA06827.1 \| U10398

Amino Acid Sequence (or in FASTA format)
1 MKTEGIKSPS AKYHDMAGSQ RIPHKNPHIQ KVAVLQSKPN KEDALNLIKE 51 IAHKVSYLMK ENHFKVTNLV EFYPRDQRLL GMNVNHGSKI MLRLRCSTDE 101 FQFLPMECIM GTMLHELTHN LFGPHDKKFY NKLDELIGRQ WVIEQRGLYD 151 TFLGNGQRLG GRANLRSNRY PMTGISTNTG IVRKRGKGVK LGSLHPEGIS 201 SIDRGNSPRE LAAFAAERRY RDDRWCGETK NNKDQIISDN ISSSLEVVIL 251 DDDDEVLPGD TLIEVIDLT*

Amino Acid Sequence (or in FASTA format)

       1  MKTEGIKSPS AKYHDMAGSQ RIPHKNPHIQ KVAVLQSKPN KEDALNLIKE
      51  IAHKVSYLMK ENHFKVTNLV EFYPRDQRLL GMNVNHGSKI MLRLRCSTDE
     101  FQFLPMECIM GTMLHELTHN LFGPHDKKFY NKLDELIGRQ WVIEQRGLYD
     151  TFLGNGQRLG GRANLRSNRY PMTGISTNTG IVRKRGKGVK LGSLHPEGIS
     201  SIDRGNSPRE LAAFAAERRY RDDRWCGETK NNKDQIISDN ISSSLEVVIL
     251  DDDDEVLPGD TLIEVIDLT*

external links for Wss1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Wss1p

1)	Biggins S, et al. (2001) Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics 159(2):453-70
2)	Hannich JT, et al. (2005) Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J Biol Chem 280(6):4102-10
3)	O'Neill BM, et al. (2004) Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response. Nucleic Acids Res 32(22):6519-30
4)	Birrell GW, et al. (2001) A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity. Proc Natl Acad Sci U S A 98(22):12608-13
5)	van Heusden GP and Steensma HY (2008) The Saccharomyces cerevisiae Wss1 protein is only present in mother cells. FEMS Microbiol Lett 282(1):100-4