UBA4/YHR111W Protein Information

Standard Name	Uba4p
Systematic Name	Yhr111wp
Alias	Yhr1p
ORF Classification	Verified
Description	Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p (1, 2, 3, 4)
Name Description	UBiquitin-like protein Activating

Experimental Data
Molecules/cell	2620 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	440
Molecular Weight (Da)	49,361
Isoelectric Point (pI)	6.5

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Uba4p (InterPro)
Physical Interactions	There are 47 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2C3 \| P38820 MIPS: YHR111W NCBI: 529119 \| 6321903 \| 731699 \| NP_011979.1 GenBank/EMBL/DDBJ: DAA06805.1 \| U00059

Amino Acid Sequence (or in FASTA format)
1 MNDYHLEDTT SELEALRLEN AQLREQLAKR EDSSRDYPLS LEEYQRYGRQ 51 MIVEETGGVA GQVKLKNTKV LVVGAGGLGC PALPYLAGAG VGQIGIVDND 101 VVETSNLHRQ VLHDSSRVGM LKCESARQYI TKLNPHINVV TYPVRLNSSN 151 AFDIFKGYNY ILDCTDSPLT RYLVSDVAVN LGITVVSASG LGTEGQLTIL 201 NFNNIGPCYR CFYPTPPPPN AVTSCQEGGV IGPCIGLVGT MMAVETLKLI 251 LGIYTNENFS PFLMLYSGFP QQSLRTFKMR GRQEKCLCCG KNRTITKEAI 301 EKGEINYELF CGARNYNVCE PDERISVDAF QRIYKDDEFL AKHIFLDVRP 351 SHHYEISHFP EAVNIPIKNL RDMNGDLKKL QEKLPSVEKD SNIVILCRYG 401 NDSQLATRLL KDKFGFSNVR DVRGGYFKYI DDIDQTIPKY *

Amino Acid Sequence (or in FASTA format)

       1  MNDYHLEDTT SELEALRLEN AQLREQLAKR EDSSRDYPLS LEEYQRYGRQ
      51  MIVEETGGVA GQVKLKNTKV LVVGAGGLGC PALPYLAGAG VGQIGIVDND
     101  VVETSNLHRQ VLHDSSRVGM LKCESARQYI TKLNPHINVV TYPVRLNSSN
     151  AFDIFKGYNY ILDCTDSPLT RYLVSDVAVN LGITVVSASG LGTEGQLTIL
     201  NFNNIGPCYR CFYPTPPPPN AVTSCQEGGV IGPCIGLVGT MMAVETLKLI
     251  LGIYTNENFS PFLMLYSGFP QQSLRTFKMR GRQEKCLCCG KNRTITKEAI
     301  EKGEINYELF CGARNYNVCE PDERISVDAF QRIYKDDEFL AKHIFLDVRP
     351  SHHYEISHFP EAVNIPIKNL RDMNGDLKKL QEKLPSVEKD SNIVILCRYG
     401  NDSQLATRLL KDKFGFSNVR DVRGGYFKYI DDIDQTIPKY *

external links for Uba4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Uba4p

1)	Goehring AS, et al. (2003) Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p. Eukaryot Cell 2(5):930-6
2)	Goehring AS, et al. (2003) Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell 14(11):4329-41
3)	Noma A, et al. (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52
4)	Leidel S, et al. (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41