GGA2/YHR108W Protein Information

Standard Name	Gga2p 1
Systematic Name	Yhr108wp
ORF Classification	Verified
Description	Protein that regulates Arf1p and Arf2p to facilitate Golgi traffickingi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication (1, 2, 3)
Name Description	Golgi-localized, Gamma-adaptin ear homology, Arf-binding protein 1

Experimental Data
Molecules/cell	2270 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	585
Molecular Weight (Da)	64,346
Isoelectric Point (pI)	6.46

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gga2p (InterPro)
Physical Interactions	There are 70 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005325C \| P38817 MIPS: YHR108W NCBI: 306440552 \| 306440553 \| 306440554 \| 529121 \| 6321900 \| 731696 \| NP_011976.1 GenBank/EMBL/DDBJ: DAA06802.1 \| U00059

Amino Acid Sequence (or in FASTA format)
1 MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA 51 DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL 101 QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND 151 MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA 201 AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK 251 ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 301 DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI 351 NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA 401 NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN 451 SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF 501 FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN 551 APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV*

Amino Acid Sequence (or in FASTA format)

       1  MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA
      51  DYINEKQGAA PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL
     101  QISRKEFLNE LVKRFPGHPP LRYSKIQRLI LTAIEEWYQT ICKHSSYKND
     151  MGYIRDMHRL LKYKGYAFPK ISESDLAVLK PSNQLKTASE IQKEQEIAQA
     201  AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI SSELNKLKRK
     251  ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ
     301  DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI
     351  NLIDFNDLDE APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA
     401  NQASFGLGGD IVLGSSQPAP PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN
     451  SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN LKIEFEISRE SNSVIRIKSF
     501  FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD GISQEGTIEN
     551  APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV*

external links for Gga2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gga2p

1)	Zhdankina O, et al. (2001) Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi. Yeast 18(1):1-18
2)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3)	Demmel L, et al. (2008) The clathrin adaptor Gga2p is a phosphatidylinositol 4-phosphate effector at the Golgi exit. Mol Biol Cell 19(5):1991-2002
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41