RPF1/YHR088W Protein Information

Standard Name	Rpf1p
Systematic Name	Yhr088wp
ORF Classification	Verified
Description	Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA (1, 2, 3, 4)

Experimental Data
Molecules/cell	784 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	295
Molecular Weight (Da)	35,121
Isoelectric Point (pI)	10.28

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpf1p (InterPro)
Physical Interactions	There are 89 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005322B \| P38805 MIPS: YHR088W NCBI: 487932 \| 6321880 \| 731684 \| NP_011956.1 GenBank/EMBL/DDBJ: DAA06784.1 \| U00060

Amino Acid Sequence (or in FASTA format)
1 MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ 51 RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNSN EPPKIFLTTN 101 VNAKKSAYEF ANILIEILPN VTFVKRKFGY KLKEISDICI KRNFTDIVII 151 NEDKKKVTGL TFIHLPEGPT FYFKLSSFVE VKKIVGHGRP TSHIPELILN 201 NFQTRLGQTV GRLFQSILPQ NPDIEGRQVI TLHNQRDYIF FRRHRYVFKD 251 NERVGLQELG PQFTLKLKRL QRGIKEETEW EHKPEMDKEK KKFYL*

Amino Acid Sequence (or in FASTA format)

       1  MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ
      51  RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNSN EPPKIFLTTN
     101  VNAKKSAYEF ANILIEILPN VTFVKRKFGY KLKEISDICI KRNFTDIVII
     151  NEDKKKVTGL TFIHLPEGPT FYFKLSSFVE VKKIVGHGRP TSHIPELILN
     201  NFQTRLGQTV GRLFQSILPQ NPDIEGRQVI TLHNQRDYIF FRRHRYVFKD
     251  NERVGLQELG PQFTLKLKRL QRGIKEETEW EHKPEMDKEK KKFYL*

external links for Rpf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rpf1p

1)	Wehner KA and Baserga SJ (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39
2)	Bogengruber E, et al. (2003) Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res 3(1):35-43
3)	Horsey EW, et al. (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4)	Caesar S, et al. (2006) Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Mol Cell Biol 26(8):3170-80
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41