IPI1/YHR085W Protein Information

Standard Name	Ipi1p 1
Systematic Name	Yhr085wp
ORF Classification	Verified
Description	Component of the Rix1 complex and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene (1, 2, 3, 4, 5)
Name Description	Involved in Processing ITS2 1

Experimental Data
Molecules/cell	4420 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	334
Molecular Weight (Da)	37,867
Isoelectric Point (pI)	10.29

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ipi1p (InterPro)
Physical Interactions	There are 50 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053233 \| P38803 MIPS: YHR085W NCBI: 500826 \| 6321877 \| 731682 \| NP_011953.1 \| NM_001179215.1 GenBank/EMBL/DDBJ: DAA06780.1 \| U10556

Amino Acid Sequence (or in FASTA format)
1 MTKSRKQKQK KQDFLRKKLK VGKPKEKARN ATDTSFVSKT ISIRNQHLDQ 51 NPHDLTKRLT LLKHHNINVR KETLTTFQKS IPSIIKSRLM TPLLTQSIPL 101 ICDESQQVRQ GLIDLVDEIG SHDAEILKLH CNIFVLYINM AMTHIVTQIQ 151 ADSTKFLSHL LKYCGDEVVR KSWVKLLNGV FGVLGWGQVG KNDSASIVQT 201 KKRNAKYVTI HLNALYTLVE YGCQDERARS DGDTAETTED SGTLRNPYLI 251 PDYPQPFEHL KLFTRELKVQ DATSSGVNAT LLSLATQDID TRKAVFIEQF 301 LPIVRKKIEV IIKEGGECGK SANKLKTLLA KIFD*

Amino Acid Sequence (or in FASTA format)

       1  MTKSRKQKQK KQDFLRKKLK VGKPKEKARN ATDTSFVSKT ISIRNQHLDQ
      51  NPHDLTKRLT LLKHHNINVR KETLTTFQKS IPSIIKSRLM TPLLTQSIPL
     101  ICDESQQVRQ GLIDLVDEIG SHDAEILKLH CNIFVLYINM AMTHIVTQIQ
     151  ADSTKFLSHL LKYCGDEVVR KSWVKLLNGV FGVLGWGQVG KNDSASIVQT
     201  KKRNAKYVTI HLNALYTLVE YGCQDERARS DGDTAETTED SGTLRNPYLI
     251  PDYPQPFEHL KLFTRELKVQ DATSSGVNAT LLSLATQDID TRKAVFIEQF
     301  LPIVRKKIEV IIKEGGECGK SANKLKTLLA KIFD*

external links for Ipi1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ipi1p

1)	Krogan NJ, et al. (2004) High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell 13(2):225-39
2)	Wu LF, et al. (2002) Large-scale prediction of Saccharomyces cerevisiae gene function using overlapping transcriptional clusters. Nat Genet 31(3):255-65
3)	Nissan TA, et al. (2004) A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol Cell 15(2):295-301
4)	Huo L, et al. (2012) The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle 11(7):1325-39
5)	Ghosh Dastidar R, et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41