SAM35/YHR083W Protein Information

Standard Name	Sam35p 1
Systematic Name	Yhr083wp
Alias	Fmp20p , Tob38p 2 , Tom38p 3
ORF Classification	Verified
Description	Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane (1, 2, 3, 4)
Name Description	Sorting and Assembly Machinery 1

Experimental Data
Molecules/cell	1470 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	329
Molecular Weight (Da)	37,404
Isoelectric Point (pI)	7.13

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sam35p (InterPro)
Physical Interactions	There are 46 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2B3 \| P14693 MIPS: YHR083W NCBI: 500828 \| 6321875 \| 731681 \| 832921 \| NP_011951.1 \| NM_001179213.1 GenBank/EMBL/DDBJ: DAA06778.1 \| U10556 \| X16112

Amino Acid Sequence (or in FASTA format)
1 MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG 51 SSELTVEGTY KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT 101 HSQEQTPSHT CNHEMLVLSR LSNPDEALPI LVEGYKKRII RSTVAISEIM 151 RSRILDDAEQ LMYYTLLDTV LYDCWITQII FCASDAQFME LYSCQKLSGS 201 IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS MQGVYHNHHN 251 SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK 301 EPIKLKTFVE NECKELVQFA QDTLKNFVQ*

Amino Acid Sequence (or in FASTA format)

       1  MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG
      51  SSELTVEGTY KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT
     101  HSQEQTPSHT CNHEMLVLSR LSNPDEALPI LVEGYKKRII RSTVAISEIM
     151  RSRILDDAEQ LMYYTLLDTV LYDCWITQII FCASDAQFME LYSCQKLSGS
     201  IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS MQGVYHNHHN
     251  SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK
     301  EPIKLKTFVE NECKELVQFA QDTLKNFVQ*

external links for Sam35p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sam35p

1)	Milenkovic D, et al. (2004) Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability. J Biol Chem 279(21):22781-5
2)	Waizenegger T, et al. (2004) Tob38, a novel essential component in the biogenesis of beta-barrel proteins of mitochondria. EMBO Rep 5(7):704-9
3)	Ishikawa D, et al. (2004) Two novel proteins in the mitochondrial outer membrane mediate beta-barrel protein assembly. J Cell Biol 166(5):621-7
4)	Habib SJ, et al. (2005) Assembly of the TOB complex of mitochondria. J Biol Chem 280(8):6434-40
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41