KSP1/YHR082C Protein Information

Standard Name	Ksp1p
Systematic Name	Yhr082cp
ORF Classification	Verified
Description	Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)

Experimental Data
Molecules/cell	1270 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,029
Molecular Weight (Da)	117,081
Isoelectric Point (pI)	6.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ksp1p (InterPro)
Physical Interactions	There are 234 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012E0D7 \| P38691 MIPS: YHR082C NCBI: 500839 \| 516233 \| 6321874 \| 729911 \| NP_011950.1 GenBank/EMBL/DDBJ: DAA06777.1 \| U10556 \| X80329
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MTLDYEIYKE GGILNNRYQK IEDISEGSYG YVSLAKDVRE KRLVAVKYIF 51 KLEDDGQYDG PQDDENDCDS SDCDDDEDTK VDTDRHENEN GNASSNNGSS 101 REKKHNLYKH KKSLISSKVK SRLSNNICLE AMYEVDIQTK IGRHQNIAAL 151 LDFFDSYIIM EYCSGGDLYE AIKADAVPKK TKSITHIITQ IMDAIEYVHN 201 KGIYHRDIKP ENILISGIDW TIKLTDWGLA TTDKTSMDRN VGSERYMSPE 251 LFDSNLDIKE RKEPYDCAKV DLWAMGIVFL NIVFHKNPFS IANQSDKSFC 301 YFAANREALF DVFSTMAYDF FQVLRYSLTI DPANRDLKMM RTELQNLSEY 351 TLDDEYYNNL DEGYEETMID GLPPQPVPPS SAPVSLPTPI SSSNKQHMPE 401 FKKDFNFNNV NERKRSDVSQ NQNVASGFFK KPSTQQQKFF NQGYNTTLST 451 HERAKSAPKF KFKKRNKYGR TDNQFSKPVN IEDRKKSKIL KKSRKPLGIP 501 TPNTHMNNFF HDYKARDEFN TRDFFTPPSV QHRYMEGFSN NNNKQYRQNR 551 NYNNNNNNSN NNHGSNYNNF NNGNSYIKGW NKNFNKYRRP SSSSYTGKSP 601 LSRYNMSYNH NNNSSINGYA RRGSTTTVQH SPGAYIPPNA RNHHVSPTNQ 651 FLRVPQSTAP DISTVLGGKP SYQEHYTQDS MDSEGDHDSD DVLFTLEEGD 701 HDFVNGMDNL SINDHLPHTT VGSHNEVFVH ASTNHNNNGN NNHIDTNSTT 751 NQYHRQYIPP PLTTSLHINN NNNESNELPD LLKSPASSEA HLNLSSGPID 801 PILTGNIGNR YSHSSDSKEL EQERRLSMEQ KFKNGVYVPP HHRKSFNLGT 851 QVPPMNMKTS NEATLSVSHN SVNFGGSYNS RRSSANESNP LHMNKALEKL 901 SSSPGAKSSF VGFPKPLLPR NHSSTTIALQ NEDVFADSNN DAIIFEDEEY 951 EGESDKMAHG KMEGGDNESS STSPDERQIF GPYEIYAQTF AGSTHDKKLG 1001 AGRKTSIQDE MVGSLEQYKN NWLILQQQD*

Amino Acid Sequence (or in FASTA format)

       1  MTLDYEIYKE GGILNNRYQK IEDISEGSYG YVSLAKDVRE KRLVAVKYIF
      51  KLEDDGQYDG PQDDENDCDS SDCDDDEDTK VDTDRHENEN GNASSNNGSS
     101  REKKHNLYKH KKSLISSKVK SRLSNNICLE AMYEVDIQTK IGRHQNIAAL
     151  LDFFDSYIIM EYCSGGDLYE AIKADAVPKK TKSITHIITQ IMDAIEYVHN
     201  KGIYHRDIKP ENILISGIDW TIKLTDWGLA TTDKTSMDRN VGSERYMSPE
     251  LFDSNLDIKE RKEPYDCAKV DLWAMGIVFL NIVFHKNPFS IANQSDKSFC
     301  YFAANREALF DVFSTMAYDF FQVLRYSLTI DPANRDLKMM RTELQNLSEY
     351  TLDDEYYNNL DEGYEETMID GLPPQPVPPS SAPVSLPTPI SSSNKQHMPE
     401  FKKDFNFNNV NERKRSDVSQ NQNVASGFFK KPSTQQQKFF NQGYNTTLST
     451  HERAKSAPKF KFKKRNKYGR TDNQFSKPVN IEDRKKSKIL KKSRKPLGIP
     501  TPNTHMNNFF HDYKARDEFN TRDFFTPPSV QHRYMEGFSN NNNKQYRQNR
     551  NYNNNNNNSN NNHGSNYNNF NNGNSYIKGW NKNFNKYRRP SSSSYTGKSP
     601  LSRYNMSYNH NNNSSINGYA RRGSTTTVQH SPGAYIPPNA RNHHVSPTNQ
     651  FLRVPQSTAP DISTVLGGKP SYQEHYTQDS MDSEGDHDSD DVLFTLEEGD
     701  HDFVNGMDNL SINDHLPHTT VGSHNEVFVH ASTNHNNNGN NNHIDTNSTT
     751  NQYHRQYIPP PLTTSLHINN NNNESNELPD LLKSPASSEA HLNLSSGPID
     801  PILTGNIGNR YSHSSDSKEL EQERRLSMEQ KFKNGVYVPP HHRKSFNLGT
     851  QVPPMNMKTS NEATLSVSHN SVNFGGSYNS RRSSANESNP LHMNKALEKL
     901  SSSPGAKSSF VGFPKPLLPR NHSSTTIALQ NEDVFADSNN DAIIFEDEEY
     951  EGESDKMAHG KMEGGDNESS STSPDERQIF GPYEIYAQTF AGSTHDKKLG
    1001  AGRKTSIQDE MVGSLEQYKN NWLILQQQD*

external links for Ksp1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ksp1p

1)	Fleischmann M, et al. (1996) Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase. Mol Gen Genet 250(5):614-25
2)	Bharucha N, et al. (2008) Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth. Mol Biol Cell 19(7):2708-17
3)	Laxman S and Tu BP (2011) Multiple TORC1-Associated Proteins Regulate Nitrogen Starvation-Dependent Cellular Differentiation in Saccharomyces cerevisiae. PLoS One 6(10):e26081
4)	Umekawa M and Klionsky DJ (2012) Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway. J Biol Chem 287(20):16300-10
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41