IRE1/YHR079C Protein Information Help

Standard Name Ire1p 1
Systematic Name Yhr079cp
Alias Ern1p
ORF Classification Verified
Description Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress (2, 3, 4)
Name Description Inositol REquiring 1
Experimental Data
Molecules/cell 259 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,115
Molecular Weight (Da) 126,974
Isoelectric Point (pI) 6.55

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Ire1p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 114 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012D881 | P32361
MIPS: YHR079C
NCBI: 166235415 | 166235416 | 218681932 | 218681933 | 218681934 | 218681935 | 218681936 | 218681937 | 218681938 | 218681939 | 218681940 | 218681941 | 218681942 | 218681943 | 218681944 | 218681945 | 295982401 | 295982402 | 295982403 | 295982404 | 295982405 | 295982406 | 339961415 | 339961416 | 339961417 | 339961418 | 339961419 | 339961420 | 339961421 | 339961422 | 339961423 | 339961424 | 339961425 | 339961426 | 339961427 | 339961428 | 339961429 | 339961430 | 339961431 | 339961432 | 339961433 | 339961434 | 339961435 | 3836 | 393281 | 500837 | 6321870 | 729857 | 85544280 | 85544281 | NP_011946.1
GenBank/EMBL/DDBJ: DAA06773.1 | L19640 | U10556 | Z11701
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
EC: 3.1.26.- [Endoribonucleases Producing 5'-Phosphomonoesters]
Amino Acid Sequence (or in FASTA format)
 
       1  MRLLRRNMLV LTLLVCVFSS IISCSIPLSS RTSRRQIVED EVASTKKLNF
      51  NYGVDKNINS PIPAPRTTEG LPNMKLSSYP TPNLLNTADN RRANKKGRRA
     101  ANSISVPYLE NRSLNELSLS DILIAADVEG GLHAVDRRNG HIIWSIEPEN
     151  FQPLIEIQEP SRLETYETLI IEPFGDGNIY YFNAHQGLQK LPLSIRQLVS
     201  TSPLHLKTNI VVNDSGKIVE DEKVYTGSMR TIMYTINMLN GEIISAFGPG
     251  SKNGYFGSQS VDCSPEEKIK LQECENMIVI GKTIFELGIH SYDGASYNVT
     301  YSTWQQNVLD VPLALQNTFS KDGMCIAPFR DKSLLASDLD FRIARWVSPT
     351  FPGIIVGLFD VFNDLRTNEN ILVPHPFNPG DHESISSNKV YLDQTSNLSW
     401  FALSSQNFPS LVESAPISRY ASSDRWRVSS IFEDETLFKN AIMGVHQIYN
     451  NEYDHLYENY EKTNSLDTTH KYPPLMIDSS VDTTDLHQNN EMNSLKEYMS
     501  PEDLEAYRKK IHEQISRELD EKNQNSLLLK FGSLVYRIIE TGVFLLLFLI
     551  FCAILQRFKI LPPLYVLLSK IGFMPEKEIP IVESKSLNCP SSSENVTKPF
     601  DMKSGKQVVF EGAVNDGSLK SEKDNDDADE DDEKSLDLTT EKKKRKRGSR
     651  GGKKGRKSRI ANIPNFEQSL KNLVVSEKIL GYGSSGTVVF QGSFQGRPVA
     701  VKRMLIDFCD IALMEIKLLT ESDDHPNVIR YYCSETTDRF LYIALELCNL
     751  NLQDLVESKN VSDENLKLQK EYNPISLLRQ IASGVAHLHS LKIIHRDLKP
     801  QNILVSTSSR FTADQQTGAE NLRILISDFG LCKKLDSGQS SFRTNLNNPS
     851  GTSGWRAPEL LEESNNLQCQ VETEHSSSRH TVVSSDSFYD PFTKRRLTRS
     901  IDIFSMGCVF YYILSKGKHP FGDKYSRESN IIRGIFSLDE MKCLHDRSLI
     951  AEATDLISQM IDHDPLKRPT AMKVLRHPLF WPKSKKLEFL LKVSDRLEIE
    1001  NRDPPSALLM KFDAGSDFVI PSGDWTVKFD KTFMDNLERY RKYHSSKLMD
    1051  LLRALRNKYH HFMDLPEDIA ELMGPVPDGF YDYFTKRFPN LLIGVYMIVK
    1101  ENLSDDQILR EFLYS*

external links for Ire1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Ire1p
1) Nikawa J and Yamashita S  (1992) IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae. Mol Microbiol 6(11):1441-6
2) Sidrauski C and Walter P  (1997) The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response. Cell 90(6):1031-9
3) Welihinda AA and Kaufman RJ  (1996) The unfolded protein response pathway in Saccharomyces cerevisiae. Oligomerization and trans-phosphorylation of Ire1p (Ern1p) are required for kinase activation. J Biol Chem 271(30):18181-7
4) Okamura K, et al.  (2000) Dissociation of Kar2p/BiP from an ER sensory molecule, Ire1p, triggers the unfolded protein response in yeast. Biochem Biophys Res Commun 279(2):445-50
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41