NMD2/YHR077C Protein Information

Standard Name	Nmd2p 1
Systematic Name	Yhr077cp
Alias	Ifs1p , Sua1p 2 , Upf2p 3 , Sup111p 4
ORF Classification	Verified
Description	Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance (1, 3, 5, 6)
Name Description	Nonsense-mediated MRNA Decay 1

Experimental Data
Molecules/cell	1280 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,089
Molecular Weight (Da)	126,746
Isoelectric Point (pI)	4.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nmd2p (InterPro)
Physical Interactions	There are 30 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013026A \| P38798 MIPS: YHR077C NCBI: 1171737 \| 500836 \| 555939 \| 6579193 \| 806704 \| 967213 \| NP_011944.2 GenBank/EMBL/DDBJ: DAA06771.1 \| U10556 \| U12137 \| U14974 \| U28158

Amino Acid Sequence (or in FASTA format)
1 MDDGRKKELH DLNTRAWNGE EVFPLKSKKL DSSIKRNTGF IKKLKKGFVK 51 GSESSLLKDL SEASLEKYLS EIIVTVTECL LNVLNKNDDV IAAVEIISGL 101 HQRFNGRFTS PLLGAFLQAF ENPSVDIESE RDELQRITRV KGNLRVFTEL 151 YLVGVFRTLD DIESKDAIPN FLQKKTGRKD PLLFSILREI LNYKFKLGFT 201 TTIATAFIKK FAPLFRDDDN SWDDLIYDSK LKGALQSLFK NFIDATFARA 251 TELHKKVNKL QREHQKCQIR TGKLRDEYVE EYDKLLPIFI RFKTSAITLG 301 EFFKLEIPEL EGASNDDLKE TASPMITNQI LPPNQRLWEN EDTRKFYEIL 351 PDISKTVEES QSSKTEKDSN VNSKNINLFF TDLEMADCKD IIDDLSNRYW 401 SSYLDNKATR NRILKFFMET QDWSKLPVYS RFIATNSKYM PEIVSEFINY 451 LDNGFRSQLH SNKINVKNII FFSEMIKFQL IPSFMIFHKI RTLIMYMQVP 501 NNVEILTVLL EHSGKFLLNK PEYKELMEKM VQLIKDKKND RQLNMNMKSA 551 LENIITLLYP PSVKSLNVTV KTITPEQQFY RILIRSELSS LDFKHIVKLV 601 RKAHWDDVAI QKVLFSLFSK PHKISYQNIP LLTKVLGGLY SYRRDFVIRC 651 IDQVLENIER GLEINDYGQN MHRISNVRYL TEIFNFEMIK SDVLLDTIYH 701 IIRFGHINNQ PNPFYLNYSD PPDNYFRIQL VTTILLNINR TPAAFTKKCK 751 LLLRFFEYYT FIKEQPLPKE TEFRVSSTFK KYENIFGNTK FERSENLVES 801 ASRLESLLKS LNAIKSKDDR VKGSSASIHN GKESAVPIES ITEDDEDEDD 851 ENDDGVDLLG EDEDAEISTP NTESAPGKHQ AKQDESEDED DEDDDEDDDD 901 DDDDDDDDGE EGDEDDDEDD DDEDDDDEEE EDSDSDLEYG GDLDADRDIE 951 MKRMYEEYER KLKDEEERKA EEELERQFQK MMQESIDARK SEKVVASKIP 1001 VISKPVSVQK PLLLKKSEEP SSSKETYEEL SKPKKIAFTF LTKSGKKTQS 1051 RILQLPTDVK FVSDVLEEEE KLKTERNKIK KIVLKRSFD*

Amino Acid Sequence (or in FASTA format)

       1  MDDGRKKELH DLNTRAWNGE EVFPLKSKKL DSSIKRNTGF IKKLKKGFVK
      51  GSESSLLKDL SEASLEKYLS EIIVTVTECL LNVLNKNDDV IAAVEIISGL
     101  HQRFNGRFTS PLLGAFLQAF ENPSVDIESE RDELQRITRV KGNLRVFTEL
     151  YLVGVFRTLD DIESKDAIPN FLQKKTGRKD PLLFSILREI LNYKFKLGFT
     201  TTIATAFIKK FAPLFRDDDN SWDDLIYDSK LKGALQSLFK NFIDATFARA
     251  TELHKKVNKL QREHQKCQIR TGKLRDEYVE EYDKLLPIFI RFKTSAITLG
     301  EFFKLEIPEL EGASNDDLKE TASPMITNQI LPPNQRLWEN EDTRKFYEIL
     351  PDISKTVEES QSSKTEKDSN VNSKNINLFF TDLEMADCKD IIDDLSNRYW
     401  SSYLDNKATR NRILKFFMET QDWSKLPVYS RFIATNSKYM PEIVSEFINY
     451  LDNGFRSQLH SNKINVKNII FFSEMIKFQL IPSFMIFHKI RTLIMYMQVP
     501  NNVEILTVLL EHSGKFLLNK PEYKELMEKM VQLIKDKKND RQLNMNMKSA
     551  LENIITLLYP PSVKSLNVTV KTITPEQQFY RILIRSELSS LDFKHIVKLV
     601  RKAHWDDVAI QKVLFSLFSK PHKISYQNIP LLTKVLGGLY SYRRDFVIRC
     651  IDQVLENIER GLEINDYGQN MHRISNVRYL TEIFNFEMIK SDVLLDTIYH
     701  IIRFGHINNQ PNPFYLNYSD PPDNYFRIQL VTTILLNINR TPAAFTKKCK
     751  LLLRFFEYYT FIKEQPLPKE TEFRVSSTFK KYENIFGNTK FERSENLVES
     801  ASRLESLLKS LNAIKSKDDR VKGSSASIHN GKESAVPIES ITEDDEDEDD
     851  ENDDGVDLLG EDEDAEISTP NTESAPGKHQ AKQDESEDED DEDDDEDDDD
     901  DDDDDDDDGE EGDEDDDEDD DDEDDDDEEE EDSDSDLEYG GDLDADRDIE
     951  MKRMYEEYER KLKDEEERKA EEELERQFQK MMQESIDARK SEKVVASKIP
    1001  VISKPVSVQK PLLLKKSEEP SSSKETYEEL SKPKKIAFTF LTKSGKKTQS
    1051  RILQLPTDVK FVSDVLEEEE KLKTERNKIK KIVLKRSFD*

external links for Nmd2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nmd2p

1)	He F and Jacobson A (1995) Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen. Genes Dev 9(4):437-54
2)	Hampsey M, et al. (1991) Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiae. Biochimie 73(12):1445-55
3)	Cui Y, et al. (1995) Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon. Genes Dev 9(4):423-36
4)	Ono B, et al. (2005) Suppression of termination mutations caused by defects of the NMD machinery in Saccharomyces cerevisiae. Genes Genet Syst 80(5):311-6
5)	He F, et al. (1997) Upf1p, Nmd2p, and Upf3p are interacting components of the yeast nonsense-mediated mRNA decay pathway. Mol Cell Biol 17(3):1580-94
6)	Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41