TRM5/YHR070W Protein Information

Standard Name	Trm5p
Systematic Name	Yhr070wp
ORF Classification	Verified
Description	tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya (1)
Name Description	Transfer RNA Methyltransferase

Experimental Data
Molecules/cell	4120 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	499
Molecular Weight (Da)	56,514
Isoelectric Point (pI)	9.35

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Trm5p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2AE \| P38793 MIPS: YHR070W NCBI: 487945 \| 6321861 \| 731672 \| NP_011937.1 \| NM_001179200.1 GenBank/EMBL/DDBJ: DAA06763.1 \| U00061
External Classifications	EC: 2.1.1.228 [tRNA (guanine(37)-N(1))-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA 51 VKFPEPKNIS VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK 101 TADGNNTPVT KGVLLHESIH SVEDAYGKLP EDALAFLKEN SAEIVPHEYV 151 LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI TGHIAHLNLR TEFKPFDSLI 201 GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL VVEQKESNCT 251 FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD 301 VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW 351 IQDEEGGKIT IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL 401 PDSAISFLGN FRGIFAAHTK GATDTIQMPW VHVHCFEKYP PGDQVTEDEL 451 HARVHARIIA ALKVTADDLP LNAVSLHLVR KVAPTKPMYC ASFQLPANV*

Amino Acid Sequence (or in FASTA format)

       1  MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA
      51  VKFPEPKNIS VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK
     101  TADGNNTPVT KGVLLHESIH SVEDAYGKLP EDALAFLKEN SAEIVPHEYV
     151  LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI TGHIAHLNLR TEFKPFDSLI
     201  GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL VVEQKESNCT
     251  FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD
     301  VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW
     351  IQDEEGGKIT IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL
     401  PDSAISFLGN FRGIFAAHTK GATDTIQMPW VHVHCFEKYP PGDQVTEDEL
     451  HARVHARIIA ALKVTADDLP LNAVSLHLVR KVAPTKPMYC ASFQLPANV*

external links for Trm5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Trm5p

1)	Bjork GR, et al. (2001) A primordial tRNA modification required for the evolution of life? EMBO J 20(1-2):231-9
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41