MED6/YHR058C Protein Information

Standard Name	Med6p 1
Systematic Name	Yhr058cp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description	MEDiator complex 1

Experimental Data
Molecules/cell	4820 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	295
Molecular Weight (Da)	32,819
Isoelectric Point (pI)	5.05

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med6p (InterPro)
Physical Interactions	There are 118 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053153 \| P38782 MIPS: YHR058C NCBI: 2098619 \| 345100890 \| 345100897 \| 345100904 \| 410563055 \| 410563062 \| 459358312 \| 487956 \| 6321849 \| 731660 \| NP_011925.1 GenBank/EMBL/DDBJ: DAA06751.1 \| U00061 \| U78080

Amino Acid Sequence (or in FASTA format)
1 MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR 51 QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY 101 MQLEEELMKL DGTEYVLSSV REPDFWVIRK QRRTNNSGVG SAKGPEIIPL 151 QDYYIIGANI YQSPTIFKIV QSRLMSTSYH LNSTLESLYD LIEFQPSQGV 201 HYKVPTDTST TATAATNGNN AGGGSNKSSV RPTGGANMAT VPSTTNVNMT 251 VNTMGTGGQT IDNGTGRTGN GNMGITTEML DKLMVTSIRS TPNYI*

Amino Acid Sequence (or in FASTA format)

       1  MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR
      51  QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY
     101  MQLEEELMKL DGTEYVLSSV REPDFWVIRK QRRTNNSGVG SAKGPEIIPL
     151  QDYYIIGANI YQSPTIFKIV QSRLMSTSYH LNSTLESLYD LIEFQPSQGV
     201  HYKVPTDTST TATAATNGNN AGGGSNKSSV RPTGGANMAT VPSTTNVNMT
     251  VNTMGTGGQT IDNGTGRTGN GNMGITTEML DKLMVTSIRS TPNYI*

external links for Med6p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Med6p

1)	Lee YC, et al. (1997) A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans. Mol Cell Biol 17(8):4622-32
2)	Myers LC, et al. (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
3)	Guglielmi B, et al. (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
4)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41