YHK8/YHR048W Protein Information

Standard Name	Yhk8p 1, 2
Systematic Name	Yhr048wp
ORF Classification	Uncharacterized
Description	Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles (2, 3, 4)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	514
Molecular Weight (Da)	57,837
Isoelectric Point (pI)	9.06

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yhk8p (InterPro)
Transmembrane Domains	There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B2A4 \| P38776 MIPS: YHR048W NCBI: 488167 \| 6321838 \| 731655 \| NP_011914.1 \| NM_001179178.1 GenBank/EMBL/DDBJ: DAA06740.1 \| U00062
External Classifications	TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]

Amino Acid Sequence (or in FASTA format)
1 MVAEFQIASA QSSALTSTEE EHCSINSDKA AKLDLELTSE RKNDGKQSHE 51 VTFNEDIADP EDIARHMSTA RRYYISSLIT FTSMVITMIS SSWTLPSTHI 101 IEHFHISHEV STLGITLYVF GLGIGPLFLS PLSELYGRRI TFLYALTLSI 151 IWQCLTIWSK TITGVMFGRF LSGFFGSAFL SVAGGAIADI FDKDQIGIPM 201 AIYTTSAFLG PSLGPIIGGA LYHQSYKWTF ITLLITSGCC LVMIIFTIPE 251 TYKPMLLIRK AKRLRKEKND QRYYAVLEVT REQTSLLSAI FLSTKRPFGL 301 LLRDRMMGVL CFYTGLELAI IYLYFVAFPY VFKKLYNFGP MEIACSYIGI 351 MVGMILSAPT CLLFQKTFEW RVKRNNGVKT PEMRFEPLFY GAFLTPVGLF 401 IFAFTCYKHV HWIAPIIGSA IFGSGVYFVF TGVFAYTVDA YRRYAASGMA 451 CNTFVRCIMA GVFPLFGLQM YKSMGVNWAG FLLAMVTVAM IPVPFLFTKY 501 GARLRAKSPY AWDD*

Amino Acid Sequence (or in FASTA format)

       1  MVAEFQIASA QSSALTSTEE EHCSINSDKA AKLDLELTSE RKNDGKQSHE
      51  VTFNEDIADP EDIARHMSTA RRYYISSLIT FTSMVITMIS SSWTLPSTHI
     101  IEHFHISHEV STLGITLYVF GLGIGPLFLS PLSELYGRRI TFLYALTLSI
     151  IWQCLTIWSK TITGVMFGRF LSGFFGSAFL SVAGGAIADI FDKDQIGIPM
     201  AIYTTSAFLG PSLGPIIGGA LYHQSYKWTF ITLLITSGCC LVMIIFTIPE
     251  TYKPMLLIRK AKRLRKEKND QRYYAVLEVT REQTSLLSAI FLSTKRPFGL
     301  LLRDRMMGVL CFYTGLELAI IYLYFVAFPY VFKKLYNFGP MEIACSYIGI
     351  MVGMILSAPT CLLFQKTFEW RVKRNNGVKT PEMRFEPLFY GAFLTPVGLF
     401  IFAFTCYKHV HWIAPIIGSA IFGSGVYFVF TGVFAYTVDA YRRYAASGMA
     451  CNTFVRCIMA GVFPLFGLQM YKSMGVNWAG FLLAMVTVAM IPVPFLFTKY
     501  GARLRAKSPY AWDD*

external links for Yhk8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	TCDB
YOGY	DIP	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Yhk8p

1)	Goffeau A, et al. (1997) Multidrug-resistant transport proteins in yeast: complete inventory and phylogenetic characterization of yeast open reading frames with the major facilitator superfamily. Yeast 13(1):43-54
2)	Barker KS, et al. (2003) Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae. J Antimicrob Chemother 51(5):1131-40
3)	Gbelska Y, et al. (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55
4)	Dias PJ, et al. (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10